Brachybacterium sp. CH-KOV3 isolated from an oil-polluted environment–a new producer of levan
| Autor: | Miroslav M. Vrvić, Jovana R. Stefanović Kojić, Branka Kekez, Inka Harju, Aleksandra Djuric, Vladimir Beškoski, Gordana Gojgić-Cvijović, Maija-Liisa Mattinen, Dragica Jakovljević |
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| Přispěvatelé: | University of Belgrade, Department of Bioproducts and Biosystems, University of Turku, Aalto-yliopisto, Aalto University |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Exopolysaccharides Sucrose Microorganism medicine.medical_treatment 010501 environmental sciences Polysaccharide ta3111 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology medicine Food science Brachybacterium paraconglomeratum Molecular Biology Flavor 0105 earth and related environmental sciences chemistry.chemical_classification Growth medium biology Prebiotic Polluted environments Pseudomonas Temperature ta1182 Fructose General Medicine Hydrogen-Ion Concentration biology.organism_classification Fructans Actinobacteria 030104 developmental biology Petroleum chemistry Environmental Pollution |
| Zdroj: | International Journal of Biological Macromolecules |
| Popis: | Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. This is peer-reviewed version of the article: Aleksandra Djurić, Gordana Gojgić-Cvijović, Dragica Jakovljević, Branka Kekez, Jovana Stefanović Kojić, Maija-Liisa Mattinen, Inka Elina Harju, Miroslav M.Vrvić, Vladimir P.Beškoski, Brachybacterium sp.CH-KOV3 isolated from an oil-polluted environment–a new producer of levan, International Journal of Biological Macromolecules, 2017, 104, 311-321, [http://dx.doi.org/10.1016/j.ijbiomac.2017.06.034] [http://cer.ihtm.bg.ac.rs/handle/123456789/2097] |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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