Identification of expressed genes in regenerating rat liver in 0-4-8-12 h short interval successive partial hepatectomy
| Autor: | Jin-Yun Yuan, Jingbo Zhang, Cui-Fang Chang, Li-Feng Zhao, Cunshuan Xu, Ke-Jin Yang, Hong-Peng Han, Hui-Yong Zhang, Wen-Qiang Li, Yu-Chang Li, Salman Rahman |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
Time Factors medicine.medical_treatment Biology Partial hepatectomy Rats Sprague-Dawley medicine Animals Hepatectomy Gene Oligonucleotide Array Sequence Analysis Gene Expression Profiling Gastroenterology General Medicine Short interval Molecular biology Liver regeneration Liver Regeneration Rats Sprague dawley Gene expression profiling Basic Research Liver Rat liver Female |
| Popis: | To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression profiles.Five hundred and fifty-one elements screened from subtractive cDNA libraries were made into a cDNA microarray (cDNA chip). Extensive gene expression analysis following 0-4-8-12 h SISPH was conducted by microarray.One hundred and eighty-three elements were selected, which were either up- or down-regulated more than 2-fold at one or more time points after SISPH. Cluster analysis and generalization analysis showed that there were five distinct temporal patterns of gene expression. Eighty-six genes were unreported, associated with liver regeneration (LR).Microarray analysis shows that the down regulated genes are much more than the up-regulated ones in SISPH; the numbers of genes expressed consistently are fewer than that expressed immediately; the genes expressed in high abundance are much fewer than that increased 2-5-fold. The comparison of SISPH with partial hepatectomy (PH) shows that the expression trends of most genes in SISPH and in PH are similar, but the expression of 43 genes is specifically altered in SISPH. |
| Databáze: | OpenAIRE |
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