Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC
| Autor: | James M. Manning, A. Seetharama Acharya, Vincent A. Fischetti, Alberto Di Donato, Belur N. Manjula |
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| Rok vydání: | 2009 |
| Předmět: |
Chromatography
Elution Fluoroacetates Hemoglobin A Reversed-phase chromatography Biochemistry Peptide Fragments Phosphates chemistry.chemical_compound chemistry Reagent Trifluoroacetic acid Trifluoroacetic Acid Histidine Trypsin Hemoglobin Acetonitrile Oligopeptides Phosphoric acid Chromatography High Pressure Liquid |
| Zdroj: | International Journal of Peptide and Protein Research. 22:78-82 |
| ISSN: | 0367-8377 |
| DOI: | 10.1111/j.1399-3011.1983.tb02071.x |
| Popis: | The tryptic peptides of the aminoethylated alpha- and beta-chain of hemoglobin have been separated on a Partisil-10 ODS-2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion-pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine-containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion-pairing of trifluoroacetate with the histidine residues of the tryptic peptides. |
| Databáze: | OpenAIRE |
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