Soy hydrolysate mimic autocrine growth factors effect of conditioned media to promote single CHO-K1 cell proliferation
| Autor: | Xu Wenyan, Sasi Bhushan, Yu Xiaoyu, Jiewei Zhang, Siddalinga Prasad, Yuan Jun, Wu Fan, H. Fai Poon, Kamal Prasad |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Autocrine growth factor Protein Hydrolysates Cell CHO Cells Biology Hydrolysate 03 medical and health sciences Cricetulus 0302 clinical medicine Conditioned medium medicine Animals Autocrine signalling Cell Proliferation 030203 arthritis & rheumatology Cloning Cell growth Cell Biology General Medicine Cell biology Autocrine Communication 030104 developmental biology medicine.anatomical_structure Cell culture Culture Media Conditioned Soybean Proteins Intercellular Signaling Peptides and Proteins Developmental Biology |
| Zdroj: | Tissue and Cell. 58:130-133 |
| ISSN: | 0040-8166 |
| DOI: | 10.1016/j.tice.2019.05.005 |
| Popis: | The increasingly competitive biopharmaceutical industry requires companies to focus on rapid and low-cost cell line development. Single-cell cloning (SCC) is a critical and high-value process for cell line development, and typically problematic because single cell proliferates slowly when cultivated at low cell densities. Conditioned media (CM) provide autocrine growth factors to facilitate single cell proliferation, thus improve SCC efficiency. However, conditioned media (CM) are not a feasible solution for industrial cell line development due to variation and cross-contamination concerns. Here, we have found an improvement in the SCC efficiency similar to CM when soy hydrolysate was supplemented in SCC media. Therefore, we concluded that hydrolysate can mimic the autocrine growth factor(s) effect to improve cloning efficiency observed in CM. |
| Databáze: | OpenAIRE |
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