CpG Oligonucleotide and Interleukin 2 stimulation enables higher cytogenetic abnormality detection rates than 12-o-tetradecanolyphorbol-13-acetate in Asian patients with B-cell chronic lymphocytic leukemia (B-CLL)
| Autor: | Tse Hui Lim, Geok Yee Lee, Alvin Soon Tiong Lim, Fiona Pui San Liaw, Lai Ching Lau, Sim Leng Tien |
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| Rok vydání: | 2014 |
| Předmět: |
Interleukin 2
Male medicine.medical_specialty Pathology Stimulation In situ hybridization Biology Sensitivity and Specificity Cytogenetics Asian People Internal medicine medicine Humans Mitosis In Situ Hybridization Fluorescence Aged Chromosome Aberrations Hematology medicine.diagnostic_test Middle Aged medicine.disease Molecular biology Leukemia Lymphocytic Chronic B-Cell Leukemia Oligodeoxyribonucleotides Interleukin-2 Tetradecanoylphorbol Acetate Female Detection rate Fluorescence in situ hybridization medicine.drug |
| Zdroj: | International journal of hematology. 100(6) |
| ISSN: | 1865-3774 |
| Popis: | The present study was designed to compare abnormality detection rates using DSP30 + IL2 and 12-O-Tetradecanoylphorbol-13-acetate (TPA) in Asian patients with B-CLL. Hematological specimens from 47 patients (29 newly diagnosed, 18 relapsed) were established as 72 h-DSP30 + IL2 and TPA cultures. Standard methods were employed to identify clonal aberrations by conventional cytogenetics (CC). The B-CLL fluorescence in situ hybridization (FISH) panel comprised ATM, CEP12, D13S25, and TP53 probes. DSP30 + IL2 cultures had a higher chromosomal abnormality detection rate (67 %) compared to TPA (44 %, p0.001). The mean number of analyzable metaphases and abnormal metaphases per slide was also higher (p0.005, p0.001, respectively). Culture success rate, percentage of complex karyotype, and percentage of non-clonal abnormal cell were not significantly different (p0.05). Thirteen cases with abnormalities were found exclusively in DSP30 + IL2 cultures compared to one found solely in TPA cultures. DSP30 + IL2 cultures were comparable to the FISH panel in detecting 11q-, +12 and 17p- but not 13q-. It also has a predilection for 11q- bearing leukemic cells compared to TPA. FISH had a higher abnormality detection rate (84.1 %) compared to CC (66.0 %) with borderline significance (p = 0.051), albeit limited by its coverage. In conclusion, DSP30 + IL2 showed a higher abnormality detection rate. However, FISH is indispensable to circumvent low mitotic indices and detect subtle abnormalities. |
| Databáze: | OpenAIRE |
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