Expression of miRNA-21 and miRNA-221 in clear cell renal cell carcinoma (ccRCC) and their possible role in the development of ccRCC
| Autor: | Kristof Harda, Katalin Gombos, Zsuzsanna Szabó, Choudhury Mahua, Tibor Flaskó, Krisztián Szegedi, Gabor Halmos |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Urology Kidney Real-Time Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Prostate microRNA Biomarkers Tumor medicine Humans RNA Neoplasm Carcinoma Renal Cell Aged Bladder cancer business.industry Gyógyszerészeti tudományok Cancer Orvostudományok Middle Aged medicine.disease Kidney Neoplasms Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs Clear cell renal cell carcinoma Cell Transformation Neoplastic 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction Oncology 030220 oncology & carcinogenesis Female Neoplasm Grading business |
| Zdroj: | Urologic Oncology: Seminars and Original Investigations. 34:533.e21-533.e27 |
| ISSN: | 1078-1439 |
| DOI: | 10.1016/j.urolonc.2016.06.011 |
| Popis: | Clear cell renal cell carcinoma (ccRCC) is the third most common urological cancer after prostate and bladder cancer but has the highest rate of mortality affecting over 40% of patients. microRNAs (miRNAs) are small noncoding RNAs that have become potential biomarkers and molecular targets for cancer treatment. Molecular markers such as miRNAs may have a role in the diagnosis of ccRCC. In this study, we examined the expressions of miRNA-21 and miRNA-221 in renal cancer patients׳ tumor and adjacent paired normal tissues investigating the possible role of these miRNAs in the development of ccRCC.Renal tumors (n = 24) and paired normal renal tissue (n = 24) samples, obtained from the Department of Urology, University of Debrecen, were analyzed for miRNA-21 and miRNA-221 expressions with quantitative real-time polymerase chain reaction.miRNA-21 and miRNA-221 expressions were significantly up-regulated in tumor specimens compared to normal tissue (P0.05). miRNA-21 and miRNA-221 showed coexpression pattern in 19 (79.2%) cases of tumor samples and 8 (33.3%) cases of paired normal renal tissues. Increased miRNA pattern showed a positive correlation with pathological status of the patients.Expression of oncogenic miRNA-21 and miRNA-221 in human ccRCC tumor tissue samples compared to adjacent nontumorous tissues might suggest that these miRNAs are involved in the development of ccRCC. |
| Databáze: | OpenAIRE |
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