Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) copolymer from sugars by recombinant Ralstonia eutropha harboring the phaC1Ps and the phaGPs genes of Pseudomonas sp. 61-3
| Autor: | Hiromi Matsusaki, Minoru Seki, Sumiko Nakae, Yoshiharu Doi, Kazunori Taguchi, Ken'ichiro Matsumoto |
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| Rok vydání: | 2001 |
| Předmět: |
Polymers and Plastics
Macromolecular Substances Polymers Polyesters Mutant Hydroxybutyrates Bioengineering Reductase Polyhydroxyalkanoates law.invention Biomaterials chemistry.chemical_compound Biosynthesis Ralstonia Bacterial Proteins law Pseudomonas Materials Chemistry Recombination Genetic biology Base Sequence equipment and supplies biology.organism_classification chemistry Biochemistry Genes Bacterial Recombinant DNA Carbohydrate Metabolism Cupriavidus necator Heterologous expression Acyltransferases Plasmids |
| Zdroj: | Biomacromolecules. 2(3) |
| ISSN: | 1525-7797 |
| Popis: | Heterologous expression of the phaGPs and the phaClPs genes encoding 3-hydroxyacyl acyl carrier protein-coenzyme A transacylase and polyhydroxyalkanoate (PHA) synthase from Pseudomonas sp. 61-3, respectively, was performed in the phbCRe negative mutant, Ralstonia eutropha PHB-4. The recombinant strain of the R. eutropha PHB-4 produced PHA copolymers consisting of 3-hydroxybutyrate (3HB) and medium-chain-length 3-hydroxyalkanoate (mcl-3HA) units of 6-12 carbon atoms from sugars. The 3HB fraction in copolymers was very high (95-97 mol%). The PHA content in the recombinant strain could further be increased by the additional introduction of the phbABRe genes from R. eutropha encoding beta-ketothiolase and NADPH-depedent acetoacetyl-coenzyme A reductase. Differential scanning calorimetry analysis of the PHA copolymers produced by the recombinant R. eutropha PHB-4 have indicated that the PHA is a random copolymer of 3HB and mcl-3HA units. |
| Databáze: | OpenAIRE |
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