VCAM-1-positive stromal cells from human bone marrow producing cytokines for B lineage progenitors and for plasma cells: SDF-1, flt3L, and BAFF
| Autor: | Roland Lauster, Wolfram Ebell, Daniel H. S. Schaumann, Jens Tuischer, Rudolf A. Manz |
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| Rok vydání: | 2006 |
| Předmět: |
Stromal cell
Transcription Genetic Immunology Plasma Cells Vascular Cell Adhesion Molecule-1 Bone Marrow Cells Plasma cell Biology B-Cell Activating Factor Lymph node stromal cell medicine Humans Cell Lineage Lymphopoiesis RNA Messenger Progenitor cell Molecular Biology B-Lymphocytes Membrane Proteins Hematopoietic Stem Cells Chemokine CXCL12 Cell biology B-1 cell Haematopoiesis medicine.anatomical_structure Cytokines Bone marrow Stromal Cells Chemokines CXC |
| Zdroj: | Molecular immunology. 44(7) |
| ISSN: | 0161-5890 |
| Popis: | The differentiation of B lymphocytes in the bone marrow is guided by the surrounding microenvironment determined by cytokines, adhesion molecules, and the extracellular matrix. These microenvironmental factors are mainly provided by stromal cells. In this paper, we report the identification of a VCAM-1-positive stromal cell population by flow cytometry. This population showed the expression of cell surface markers known to be present on stromal cells (CD10, CD13, CD90, CD105) and had a fibroblastoid phenotype in vitro. Single cell RT-PCR analysis of its cytokine expression pattern revealed transcripts for haematopoietic cytokines important for either the early B lymphopoiesis like flt3L or the survival of long-lived plasma cells like BAFF or both processes like SDF-1. Whereas SDF-1 transcripts were detectable in all VCAM-1-positive cells, flt3L and BAFF were only expressed by some cells suggesting the putative existence of different subpopulations with distinct functional properties. In summary, the VCAM-1-positive cell population seems to be a candidate stromal cell population supporting either developing B cells and/or long-lived plasma cells in human bone marrow. |
| Databáze: | OpenAIRE |
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