Activity-Based Protein Profiling (ABPP) for cellular deubiquitinase (DUB) and inhibitor profiling at deep and high-throughput levels
| Autor: | Hannah B. L. Jones, Raphael Heilig, Benedikt M. Kessler, Adán Pinto-Fernández |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071628027 |
| Popis: | This chapter provides detailed methodology and materials required to profile deubiquitinases (DUBs) in a cellular matrix using specific activity-based probes, with immunoblotting and mass spectrometry proteomics-based readouts. Different types of activity-based protein profiling (ABPP) for studying the potency and selectivity of DUB inhibitors are outlined here, including the standard ABPP, the deep DUBome ABPP, and the ABPP-HT (high-throughput compatible). |
| Databáze: | OpenAIRE |
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