Overexpression of Arabidopsis miR157b induces bushy architecture and delayed phase transition in Torenia fournieri
| Autor: | Katsutomo Sasaki, Norihiro Ohtsubo, Hiroyasu Yamaguchi, Masahito Shikata |
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| Rok vydání: | 2012 |
| Předmět: |
Small RNA
DNA Complementary Molecular Sequence Data Arabidopsis Down-Regulation Gene Expression Flowers Plant Science Real-Time Polymerase Chain Reaction Magnoliopsida Gene Expression Regulation Plant microRNA Botany Genetics RNA Messenger Gene Phylogeny Plant Proteins Torenia fournieri Molecular breeding Messenger RNA Base Sequence biology Gene Expression Regulation Developmental food and beverages Sequence Analysis DNA Plants Genetically Modified biology.organism_classification Plant Leaves MicroRNAs Phenotype Torenia RNA Plant Transcription Factors |
| Zdroj: | Planta. 236:1027-1035 |
| ISSN: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-012-1649-3 |
| Popis: | miR156/157 is a small RNA molecule that is highly conserved among various plant species. Overexpression of miR156/157 has been reported to induce bushy architecture and delayed phase transition in several plant species. To investigate the effect of miR157 overexpression in a horticultural plant, and to explore the applicability of miRNA to molecular breeding, we introduced Arabidopsis MIR157b (AtMIR157b) into torenia (Torenia fournieri). The resulting 35S:AtMIR157b plants showed a high degree of branching along with small leaves, which resembled miR156/157-overexpressing plants of other species. We also isolated torenia SBP-box genes with target miR156/157 sequences and confirmed that their expression was selectively downregulated in 35S:AtMIR157b plants. The reduced accumulation of mRNA was probably due to sequence specificity. Moreover, expression of torenia homologs of the SBP-box protein-regulated genes TfLFY and TfMIR172 was also reduced by AtmiR157 overexpression. These findings suggest that the molecular mechanisms of miR156/157 regulation are conserved between Arabidopsis and torenia. The bushy architecture and small leaves of 35S:AtMIR157b torenia plants could be applied in molecular breeding of various horticultural plants as well as for increasing biomass and crop production. |
| Databáze: | OpenAIRE |
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