Surface Proteome of Plasma Extracellular Vesicles as Biomarkers for Pneumonia and Acute Exacerbation of Chronic Obstructive Pulmonary Disease
| Autor: | Rikke Bæk, Malene Jørgensen, Rembert Koczulla, Kristina Auf Dem Brinke, Wilhelm Bertrams, Stefan Hippenstiel, Nina Timmesfeld, Timm Greulich, Norbert Suttorp, Anna Lena Jung, M Han, Bernd Schmeck, Kathrin Griss |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Acute exacerbation of chronic obstructive pulmonary disease Proteome exosomes EV Array Cohort Studies Diagnosis Differential 03 medical and health sciences Extracellular Vesicles Pulmonary Disease Chronic Obstructive 0302 clinical medicine Community-acquired pneumonia COPD pneumonia Immunology and Allergy Medicine Humans acute exacerbation plasma CD63 business.industry Area under the curve Extracellular vesicle Pneumonia medicine.disease 030104 developmental biology Infectious Diseases 030228 respiratory system Case-Control Studies Immunology Disease Progression biomarker Biomarker (medicine) extracellular vesicles business Biomarkers |
| Zdroj: | Jung, A L, Møller Jørgensen, M, Bæk, R, Griss, K, Han, M, Auf Dem Brinke, K, Timmesfeld, N, Bertrams, W, Greulich, T, Koczulla, R, Hippenstiel, S, Suttorp, N & Schmeck, B 2020, ' Surface proteome of plasma extracellular vesicles as biomarkers for pneumonia and acute exacerbation of chronic obstructive pulmonary disease ', The Journal of Infectious Diseases, vol. 221, no. 2, pp. 325-335 . https://doi.org/10.1093/infdis/jiz460 |
| ISSN: | 1537-6613 |
| DOI: | 10.1093/infdis/jiz460 |
| Popis: | Background Community-acquired pneumonia (CAP) and acute exacerbation of chronic obstructive pulmonary disease (AECOPD) represent a major burden of disease and death and their differential diagnosis is critical. A potential source of relevant accessible biomarkers are blood-borne small extracellular vesicles (sEVs). Methods We performed an extracellular vesicle array to find proteins on plasma sEVs that are differentially expressed and possibly allow the differential diagnosis between CAP and AECOPD. Plasma samples were analyzed from 21 healthy controls, 24 patients with CAP, and 10 with AECOPD . The array contained 40 antibodies to capture sEVs, which were then visualized with a cocktail of biotin-conjugated CD9, CD63, and CD81 antibodies. Results We detected significant differences in the protein decoration of sEVs between healthy controls and patients with CAP or AECOPD. We found CD45 and CD28 to be the best discrimination markers between CAP and AECOPD in receiver operating characteristic analyses, with an area under the curve >0.92. Additional ensemble feature selection revealed the possibility to distinguish between CAP and AECOPD even if the patient with CAP had COPD, with a panel of CD45, CD28, CTLA4 (cytotoxic T-lymphocyte-associated protein 4), tumor necrosis factor–R-II, and CD16. Conclusion The discrimination of sEV-associated proteins is a minimally invasive method with potential to discriminate between CAP and AECOPD. |
| Databáze: | OpenAIRE |
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