Recombination is a key driver of genomic and phenotypic diversity in a Pseudomonas aeruginosa population during cystic fibrosis infection

Autor: Alan McNally, Stephen P. Diggle, Shanika A. Crusz, Sophie E. Darch, Stephen Holden, Jukka Corander, Helen L. Barr, Andrew W. Fogarty, Freya Harrison, Konrad Paszkiewicz
Přispěvatelé: Department of Mathematics and Statistics, Biostatistics Helsinki
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Cystic Fibrosis
Genotyping Techniques
medicine.disease_cause
Genotype
Phylogeny
1183 Plant biology
microbiology
virology
Recombination
Genetic
Genetics
0303 health sciences
education.field_of_study
Multidisciplinary
Quorum Sensing
Drug Resistance
Microbial
Anti-Bacterial Agents
3. Good health
Phenotype
ESCHERICHIA-COLI
LUNG INFECTIONS
Pseudomonas aeruginosa
Female
SPREAD
TRANSMISSION
education
Population
Virulence
Single-nucleotide polymorphism
Microbial Sensitivity Tests
Biology
TUBERCULOSIS
Polymorphism
Single Nucleotide
Article
Microbiology
Evolution
Molecular
Young Adult
03 medical and health sciences
Genetic variation
medicine
Humans
Pseudomonas Infections
N-ACYLHOMOSERINE LACTONES
Gene
030304 developmental biology
BACTERIAL GENOMES
Whole genome sequencing
030306 microbiology
Sputum
Genetic Variation
EPIDEMIC STRAIN
EVOLUTION
INDIVIDUALS
Mutation
Sequence Alignment
Genome
Bacterial
Zdroj: Scientific Reports
ISSN: 2045-2322
Popis: The Cystic Fibrosis (CF) lung harbors a complex, polymicrobial ecosystem, in which Pseudomonas aeruginosa is capable of sustaining chronic infections, which are highly resistant to multiple antibiotics. Here, we investigate the phenotypic and genotypic diversity of 44 morphologically identical P. aeruginosa isolates taken from a single CF patient sputum sample. Comprehensive phenotypic analysis of isolates revealed large variances and trade-offs in growth, virulence factors and quorum sensing (QS) signals. Whole genome analysis of 22 isolates revealed high levels of intra-isolate diversity ranging from 5 to 64 SNPs and that recombination and not spontaneous mutation was the dominant driver of diversity in this population. Furthermore, phenotypic differences between isolates were not linked to mutations in known genes but were statistically associated with distinct recombination events. We also assessed antibiotic susceptibility of all isolates. Resistance to antibiotics significantly increased when multiple isolates were mixed together. Our results highlight the significant role of recombination in generating phenotypic and genetic diversification during in vivo chronic CF infection. We also discuss (i) how these findings could influence how patient-to-patient transmission studies are performed using whole genome sequencing and (ii) the need to refine antibiotic susceptibility testing in sputum samples taken from patients with CF.
Databáze: OpenAIRE
Externí odkaz: