Long non-coding RNA DLGAP1-AS1 promotes the progression of gastric cancer via miR-515-5p/MARK4 axis
| Autor: | Li, Liping, Lai, Qingjun, Zhang, Manman, Jia, Jun |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Medicine (General) QH301-705.5 Physiology Proliferation Immunology Biophysics Ocean Engineering Protein Serine-Threonine Kinases Biology medicine.disease_cause Biochemistry 03 medical and health sciences R5-920 0302 clinical medicine Cell Movement Stomach Neoplasms MARK4 Cell Line Tumor medicine Humans Biology (General) General Pharmacology Toxicology and Pharmaceutics Regulation of gene expression Gene knockdown Cell growth General Neuroscience miR-515-5p Cancer RNA Cell Biology General Medicine medicine.disease Long non-coding RNA Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Cancer research RNA Long Noncoding Signal transduction Gastric cancer DLGAP1-AS1 Carcinogenesis Research Article |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 54, Iss 8 (2021) Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research, Volume: 54, Issue: 8, Article number: e10062, Published: 24 MAY 2021 Brazilian Journal of Medical and Biological Research v.54 n.8 2021 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| ISSN: | 1414-431X 0100-879X |
| Popis: | Long non-coding RNA (lncRNA) is an essential regulator of carcinogenesis and cancer progression. In the study, we explored the role of lncRNA DLGAP1-AS1 in gastric cancer (GC). qRT-PCR was carried out to detect DLGAP1-AS1 expression in GC tissues and cell lines. CCK-8 assay, EdU assay, and transwell experiments were employed to detect the malignant biological behaviors of GC cells with DLGAP1-AS1 knockdown or overexpression. Bioinformatics and dual-luciferase report assay were used to confirm the binding relationship between DLGAP1-AS1 and miR-515-5p. MARK4 expression was detected by western blot after DLGAP1-AS1/miR-515-5p was selectively regulated. DLGAP1-AS1 was up-regulated in GC tissues and cell lines, and its high expression was closely associated with larger tumor size, higher TNM stage, and lymph node metastasis. Furthermore, DLGAP1-AS1 overexpression enhanced cell proliferation, migration, and invasion, and miR-515-5p could reverse these effects. DLGAP1-AS1 participated in the regulation of the MARK4 signaling pathway by targeting miR-515-5p. DLGAP1-AS1 promoted GC progression through miR-515-5p/MARK4 signaling pathway. |
| Databáze: | OpenAIRE |
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