Unraveling the differential expression of the two isoforms of myelin-associated glycoprotein in a mouse expressing GFP-tagged S-MAG specifically regulated and targeted into the different myelin compartments
| Autor: | Bettina Flueck, Nicole Schaeren-Wiemers, Beat Erne, Andreas J. Steck, Michael Erb, Frances Kern |
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| Rok vydání: | 2005 |
| Předmět: |
Gene isoform
Nervous system Proteolipid protein 1 Recombinant Fusion Proteins Green Fluorescent Proteins Mice Transgenic Biology Models Biological Cellular and Molecular Neuroscience Myelin Mice Compact myelin medicine Animals Protein Isoforms Peripheral Nerves RNA Messenger Axon Molecular Biology Myelin Sheath Myelin-associated glycoprotein Brain Gene Expression Regulation Developmental Cell Biology Molecular biology Sciatic Nerve Axons Cell biology Mice Inbred C57BL Myelin-Associated Glycoprotein medicine.anatomical_structure nervous system Myelinogenesis |
| Zdroj: | Molecular and cellular neurosciences. 31(4) |
| ISSN: | 1044-7431 |
| Popis: | The two myelin-associated glycoprotein (MAG) isoforms are cell adhesion molecules that differ only in their cytoplasmic domains, but their specific roles are not well understood. In this study, we present a transgenic mouse line that specifically expresses GFP-tagged S-MAG correctly regulated and targeted into the myelin sheath allowing the specific discrimination of L- and S-MAG on the subcellular level. Here, we describe the differential expression pattern and spatial distribution of L- and S-MAG during development as well as in the adult central and peripheral nervous system. In peripheral nerves, where S-MAG is the sole isoform, we observed S-MAG concentrated in different ring-like structures such as periaxonal and abaxonal rings, and discs spanning through the compact myelin sheath perpendicular to the axon. In summary, our data provide new insight in the subcellular distribution of the two isoforms fundamental for the understanding of their specific functions in myelin formation and maintenance. |
| Databáze: | OpenAIRE |
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