Fluidity state of lymphocyte plasma membrane in malignant hyperthermia susceptible pigs and humans

Autor: X. Vignon, C. Motta, E. Rock, G. Kozak‐Ribbens
Přispěvatelé: Station de recherches sur la viande, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration
Jazyk: angličtina
Rok vydání: 1993
Předmět:
Pathology
medicine.medical_specialty
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering
Membrane Fluidity
Swine
Lymphocyte
03 medical and health sciences
0302 clinical medicine
030202 anesthesiology
[SDV.IDA]Life Sciences [q-bio]/Food engineering
medicine
Animals
Humans
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Lymphocytes
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
Ryanodine receptor
business.industry
Endoplasmic reticulum
Ligand binding assay
Cell Membrane
Malignant hyperthermia
General Medicine
[SDV.IDA] Life Sciences [q-bio]/Food engineering
medicine.disease
3. Good health
Anesthesiology and Pain Medicine
medicine.anatomical_structure
Membrane
Caffeine contracture
Disease Susceptibility
Halothane
business
Malignant Hyperthermia
medicine.drug
Zdroj: Acta Anaesthesiologica Scandinavica
Acta Anaesthesiologica Scandinavica, Wiley, 1993, 37, pp.488-492
ISSN: 0001-5172
1399-6576
Popis: Recent studies suggest that abnormalities occur at the lipid level in malignant hyperthermia susceptible humans and pigs. To test this hypothesis, we first investigated the physical state of plasma membranes of lymphocytes isolated from normal and malignant hyperthermia susceptible swine. In halothane-challenged pigs, malignant hyperthermia susceptibility was also assessed by ryanodine binding assay on purified sarcoplasmic reticulum membranes. The results clearly show that plasma membrane of lymphocytes from malignant hyperthermic pigs are significantly more fluid than controls. We then attempted to apply the same methodology to lymphocytes prepared from human patients previously diagnosed by the halothane and caffeine contracture test. In that case, there was no clear relationship between malignant hyperthermia susceptibility and the fluidity state of lymphocyte plasma membranes.
Databáze: OpenAIRE
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