Development and validation of High-performance Thin-layer Chromatography Method for Simultaneous Determination of Polyphenolic Compounds in Medicinal Plants
| Autor: | Sayeed Ahamad, C V Jayachandran Nair, Washim Khan, Varisha Anjum, Rajani Mathur |
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| Rok vydání: | 2018 |
| Předmět: |
Aegle marmelos
Formic acid Ethyl acetate Context (language use) 030226 pharmacology & pharmacy 01 natural sciences quercetin 03 medical and health sciences chemistry.chemical_compound Rutin 0302 clinical medicine Drug Discovery Gallic acid High performance thin layer chromatography Pharmacology Detection limit Chromatography 010401 analytical chemistry rutin 0104 chemical sciences Psidium guajava chemistry Original Article gallic acid Quercetin high-performance thin-layer chromatography |
| Zdroj: | Pharmacognosy Research |
| ISSN: | 0976-4836 |
| Popis: | Context: Quantitative standardization of plant-based products is challenging albeit essential to maintain their quality. Aims: This study aims to develop and validate high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination of rutin (Ru), quercetin (Qu), and gallic acid (Ga) from Psidium guajava Linn. (PG) and Aegle marmelos (L.) Correa. (AM) and correlate with antioxidant activity. Materials and Methods: The stock solution (1 mg/mL) of standard Ru, Qu, and Ga in methanol: Water (1:1) was serially diluted and spotted (5 μL) on slica gel 60 F254 thin-layer chromatography plates. Toluene: Ethyl acetate: Formic acid: Methanol (3:4:0.8:0.7, v/v/v) was selected as mobile phase for analysis at 254 nm. Hydroalcoholic (1:1) extracts of leaves of PG and AM were fractionated and similarly analyzed. Antioxidant activity was also determined using 2, 2-diphenyl-1-picrylhydrazyl assay. Results: The developed method was robust and resolved Ru, Qu, and Ga at Rf 0.08 ± 0.02, 0.76 ± 0.01, and 0.63 ± 0.02, respectively. The intra-day, interday precision, and interanalyst were |
| Databáze: | OpenAIRE |
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