Loss of transforming growth factor-beta 2 leads to impairment of central synapse function
| Autor: | Frederique Varoqueaux, Vardanush Sargsyan, Michael Rickmann, Weiqi Zhang, Kerstin Krieglstein, Katharina Heupel, Jaap J. Plomp |
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| Rok vydání: | 2008 |
| Předmět: |
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Western Diaphragm Neuromuscular Junction Synaptogenesis Neurotransmission Synaptic Transmission lcsh:RC346-429 Neuromuscular junction Synapse Mice Transforming Growth Factor beta2 03 medical and health sciences Glutamatergic 0302 clinical medicine Developmental Neuroscience Pregnancy medicine Biological neural network Animals lcsh:Neurology. Diseases of the nervous system 030304 developmental biology Mice Knockout Neurons 0303 health sciences Microscopy Confocal biology Miniature Postsynaptic Potentials Respiratory center biology.organism_classification Immunohistochemistry Electrophysiology Plethysmography Microscopy Electron medicine.anatomical_structure Aplysia Synapses Female Neuroscience 030217 neurology & neurosurgery Brain Stem Research Article |
| Zdroj: | Neural Development Neural Development, Vol 3, Iss 1, p 25 (2008) |
| ISSN: | 1749-8104 |
| DOI: | 10.1186/1749-8104-3-25 |
| Popis: | Background The formation of functional synapses is a crucial event in neuronal network formation, and with regard to regulation of breathing it is essential for life. Members of the transforming growth factor-beta (TGF-β) superfamily act as intercellular signaling molecules during synaptogenesis of the neuromuscular junction of Drosophila and are involved in synaptic function of sensory neurons of Aplysia. Results Here we show that while TGF-β2 is not crucial for the morphology and function of the neuromuscular junction of the diaphragm muscle of mice, it is essential for proper synaptic function in the pre-Bötzinger complex, a central rhythm organizer located in the brainstem. Genetic deletion of TGF-β2 in mice strongly impaired both GABA/glycinergic and glutamatergic synaptic transmission in the pre-Bötzinger complex area, while numbers and morphology of central synapses of knock-out animals were indistinguishable from their wild-type littermates at embryonic day 18.5. Conclusion The results demonstrate that TGF-β2 influences synaptic function, rather than synaptogenesis, specifically at central synapses. The functional alterations in the respiratory center of the brain are probably the underlying cause of the perinatal death of the TGF-β2 knock-out mice. |
| Databáze: | OpenAIRE |
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