Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats
| Autor: | Maria Raquel Marçal Natali, Silvano Piovan, Carlos Vinícius Dalto da Rosa, Giuliana Maria Ledesma Peixoto, Silvio Claudio da Costa, Maria Rosa Trentin Zorzenon, Cecília Edna Mareze-Costa, Paulo Cezar de Freitas Mathias, Yago Carvalho Lima, Paula Gimenez Milani |
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| Přispěvatelé: | Universidade Estadual de Maringá (UEM), Universidade Estadual Paulista (Unesp), Univ North Parana |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Whey protein Supplementation medicine.medical_treatment 030209 endocrinology & metabolism Fraction (chemistry) 03 medical and health sciences 0302 clinical medicine Internal medicine Diabetes mellitus parasitic diseases medicine Reduction of hyperglycemia 030109 nutrition & dietetics Chemistry Insulin Insulin secretion Diabetes food and beverages Fraction of ethyl acetate Radioimmunoassay medicine.disease Streptozotocin Stevia rebaudiana Endocrinology Immunohistochemistry Food Science medicine.drug |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2021-06-25T11:21:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-25 The study aims to analyse the treatment of whey protein enriched withStevia rebaudianafraction in insulin secretion and its role mitigating streptozotocin-induced hyperglycemia in rats. Thus, diabetic animals were treated with whey protein enriched withS. rebaudianafraction or with only the protein isolate or only the Stevia fraction. Insulin level in plasma was measured by radioimmunoassay and the viability of beta cells was detected by immunohistochemistry. The results showed that diabetic animals treated with whey protein enriched withS. rebaudianafraction had a greater recovery from insulinemia, with plasma levels similar to non-diabetic animals (similar to 0.13 ng/mL). In addition, the same group showed a higher number of insulin-positive pancreatic B cells (similar to 66%) in immunohistochemistry analysis, while the diabetic groups treated with only the fraction of stevia or whey protein showed 38 and 59% of positive cells, respectively. These results show that the treatment may have restored the viability of streptozotocin-injured pancreatic B cells, and consequently increased insulin secretion, suggesting whey protein enriched withS. rebaudianafraction can be used an adjunct/supplement in diabetic treatment. Univ Estadual Maringa, Dept Biochem, Maringa, PR, Brazil Univ Estadual Maringa, Dept Biotechnol Genet & Cell Biol, Maringa, PR, Brazil State Univ Sao Paulo, Postgrad Program Sci Human Physiol, Sao Paulo, SP, Brazil Univ North Parana, UNOPAR, Bandeirantes, PR, Brazil Univ Estadual Maringa, Dept Physiol Sci, Maringa, PR, Brazil Univ Estadual Maringa, Dept Morphol Sci, Maringa, PR, Brazil State Univ Sao Paulo, Postgrad Program Sci Human Physiol, Sao Paulo, SP, Brazil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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