Zinc Modulates the Response to Apoptosis in an In Vitro Model with High Glucose and Inflammatory Stimuli in C2C12 Cells
| Autor: | Manuel Ruz, Miguel Arredondo-Olguín, Mónica Andrews-Guzmán |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Programmed cell death Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry bcl-X Protein Caspase 3 Apoptosis 010501 environmental sciences 01 natural sciences Biochemistry Inorganic Chemistry 03 medical and health sciences Internal medicine medicine Myocyte Gene 0105 earth and related environmental sciences bcl-2-Associated X Protein 0303 health sciences Chemistry Insulin 030302 biochemistry & molecular biology Biochemistry (medical) General Medicine XIAP Zinc Endocrinology Glucose Proto-Oncogene Proteins c-bcl-2 C2C12 |
| Zdroj: | Biological trace element research. 199(6) |
| ISSN: | 1559-0720 |
| Popis: | Apoptosis is programmed cell death and its alteration is related to cancer, neurologic, autoimmune, and chronic diseases. A number of factors can affect this process. The aim of this paper is to study the effect of supplemental zinc on apoptosis-related genes in C2C12 myoblast cells after being challenged with a series of stimuli, such as high glucose, insulin, and an inflammatory agent. C2C12 myoblast cells were cultured for 24 h with zinc (Zn) (ZnSO4) 10 or 100 μM and/or glucose 10 or 30 mM. In addition to these stimuli, the cells were challenged with insulin 1 nM or interleukin-6 (IL-6) 5 nM. The mRNA expression of proapoptotic genes caspase 3 and Fas, the antiapoptotic genes, Xiap and Bcl-xL and the ratio of pro-/antiapoptotic genes Bax/Bcl-2, were determined by qRT-PCR. The expression of caspase-3 gene was significantly increased in the presence of the combination high Zn/high glucose with and without the presence of insulin and IL6 in the culture medium Fas expression instead, showed uneven responses. The expression of Bcl-xL and Xiap was increased in most conditions by having high Zn in the medium regardless of the presence of insulin or IL6. Bax/Bcl2 ratio was decreased in the presence of high Zn. Zn was able to stimulate the expression of antiapoptotic genes. This effect was specially noted in high-glucose conditions with and without the presence of insulin. This effect is partially overridden by the presence of an inflammatory agent such as IL-6. |
| Databáze: | OpenAIRE |
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