Mechanics and kinetics of dynamic instability
| Autor: | Michaels, Thomas Ct, Feng, Shuo, Liang, Haiyi, Mahadevan, L |
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| Přispěvatelé: | Michaels, Thomas Ct [0000-0001-6931-5041], Feng, Shuo [0000-0003-0530-8381], Liang, Haiyi [0000-0001-7458-8036], Mahadevan, L [0000-0002-5114-0519], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Models Molecular GTP' QH301-705.5 none Science Protein subunit Kinetics macromolecular substances Kinetic energy Instability Guanosine Diphosphate Microtubules General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Microtubule Tubulin physics of living systems Computer Simulation Biology (General) General Immunology and Microbiology biology Chemistry General Neuroscience dynamic instability General Medicine Statistical mechanics 030104 developmental biology biology.protein Biophysics Medicine Guanosine Triphosphate 030217 neurology & neurosurgery Research Article microtubule Protein Binding |
| Zdroj: | eLife eLife, Vol 9 (2020) |
| Popis: | During dynamic instability, self-assembling microtubules (MTs) stochastically alternate between phases of growth and shrinkage. This process is driven by the presence of two distinct states of MT subunits, GTP- and GDP-bound tubulin dimers, that have different structural properties. Here, we use a combination of analysis and computer simulations to study the mechanical and kinetic regulation of dynamic instability in three-dimensional (3D) self-assembling MTs. Our model quantifies how the 3D structure and kinetics of the distinct states of tubulin dimers determine the mechanical stability of MTs. We further show that dynamic instability is influenced by the presence of quenched disorder in the state of the tubulin subunit as reflected in the fraction of non-hydrolysed tubulin. Our results connect the 3D geometry, kinetics and statistical mechanics of these tubular assemblies within a single framework, and may be applicable to other self-assembled systems where these same processes are at play. |
| Databáze: | OpenAIRE |
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