Foxm1 regulates neural progenitor fate during spinal cord regeneration
| Autor: | Syed Murtuza Baker, Lauren S. Phipps, Raphael Thuret, Carlos J Gallardo-Dodd, Diane Pelzer, Karel Dorey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Spinal Cord Regeneration
Xenopus Regenerative Medicine Biochemistry Article Xenopus laevis Foxm1 Genetics medicine Animals Progenitor cell Molecular Biology Transcription factor Spinal Cord Injuries Progenitor biology Regeneration (biology) spinal cord Articles differentiation scRNAseq Spinal cord Regenerative process biology.organism_classification xenopus Cell biology medicine.anatomical_structure Gene Expression Regulation Larva regeneration foxm1 Development & Differentiation Neuroscience |
| Zdroj: | Pelzer, D, Phipps, L S, Thuret, R, Gallardo-Dodd, C J, Baker, S M & Dorey, K 2021, ' Foxm1 regulates neural progenitor fate during spinal cord regeneration ', EMBO reports, vol. 22, no. 9, e50932 . https://doi.org/10.15252/embr.202050932 EMBO Reports |
| Popis: | Xenopus tadpoles have the ability to regenerate their tails upon amputation. Although some of the molecular and cellular mechanisms that globally regulate tail regeneration have been characterised, tissue‐specific response to injury remains poorly understood. Using a combination of bulk and single‐cell RNA sequencing on isolated spinal cords before and after amputation, we identify a number of genes specifically expressed in the spinal cord during regeneration. We show that Foxm1, a transcription factor known to promote proliferation, is essential for spinal cord regeneration. Surprisingly, Foxm1 does not control the cell cycle length of neural progenitors but regulates their fate after division. In foxm1 −/− tadpoles, we observe a reduction in the number of neurons in the regenerating spinal cord, suggesting that neuronal differentiation is necessary for the regenerative process. Altogether, our data uncover a spinal cord‐specific response to injury and reveal a new role for neuronal differentiation during regeneration. Differentiation of neural progenitor cells (NPCs) is a hallmark of successful spinal cord regeneration. This study shows that Foxm1 controls the switch from proliferation to differentiation of NPCs during spinal cord regeneration in Xenopus tropicalis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|