High efficacy of ozonated oils on the removal of biofilms produced by methicillin-resistant staphylococcus aureus (MRSA) from infected diabetic foot ulcers
| Autor: | Rosa Capita, Carlos Alonso-Calleja, Águeda Carvalho, José Eduardo Pereira, José A Marques-Magallanes, Gilberto Igrejas, Cecília Peirone, Vanessa Silva, Luís Maltez, Joana S. Amaral, Ângela Martins, José Luis Capelo, Patrícia Poeta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
medicine.drug_class
Antibiotics Ozonated oil Pharmaceutical Science diabetic foot ulcers MRSA medicine.disease_cause Analytical Chemistry lcsh:QD241-441 03 medical and health sciences Ozone lcsh:Organic chemistry Drug Discovery medicine Peroxide value Food science Agar diffusion test Physical and Theoretical Chemistry Unsaturated fatty acid 030304 developmental biology chemistry.chemical_classification 0303 health sciences 030306 microbiology Chemistry Organic Chemistry Biofilm Fatty acid Antimicrobial S. aureus Methicillin-resistant Staphylococcus aureus ozone ozonated oil Chemistry (miscellaneous) Biofilms Diabetic foot ulcers Molecular Medicine biofilms |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Molecules Volume 25 Issue 16 Molecules, Vol 25, Iss 3601, p 3601 (2020) |
| Popis: | Ozone has a high wound healing capacity and antibacterial properties and can be used as a complementary treatment in infections. Methicillin-resistant S. aureus (MRSA) is the most common pathogen found in infected diabetic foot ulcers. Most of MRSA are resistant to several classes of antibiotics and, therefore, there is a need for new, effective, and well-tolerated agents. Thus, we aimed evaluate the antimicrobial and antibiofilm potentials of ozonated vegetable oils against MRSA strains isolated from diabetic foot ulcers. Six ozonated oils were produced with concentrations of ozone ranging from 0.53 to 17 mg of ozone/g of oil. The peroxide values were determined for each oil. Ozonated oils content on fatty acid was determined by gas chromatography equipped with a flame ionization detector. The antimicrobial susceptibility testing was performed by the Kirby&ndash Bauer disk diffusion method and the effect of ozonated oils on biofilm formation ability and on established biofilms was investigated. In general, the content in identified unsaturated fatty acid in oils decreased with the increase of ozonation time and, consequently, the peroxide value increased. Most bacterial strains were inhibited by ozonated oil at a concentration of 4.24 mg/g. Ozonated oils had moderate to high ability to remove adhered cells and showed a high capacity to eradicate 24 h old biofilms. Our results show promising use of ozonated oils on the treatment of infections, in particular those caused by multidrug-resistant MRSA strains. |
| Databáze: | OpenAIRE |
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