The ferret: a new model of oral ethanol injury involving the liver, bone marrow, and peripheral blood lymphocytes
| Autor: | Gary A. Roselle, Albert F. Muhleman, Anthony Chedid, Charles L. Mendenhall |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
medicine.medical_specialty
Liquid diet Myeloid Carnivora Leukocyte Migration-Inhibitory Factors Medicine (miscellaneous) Biology Toxicology Lymphocyte Activation chemistry.chemical_compound Bone Marrow Internal medicine medicine Animals Lymphocytes Ethanol Acetaldehyde Ferrets Blood Cell Count Psychiatry and Mental health Alcoholism Disease Models Animal medicine.anatomical_structure Endocrinology chemistry Vacuolization Liver Immunology Hepatic stellate cell Bone marrow |
| Zdroj: | Alcoholism, clinical and experimental research. 10(3) |
| ISSN: | 0145-6008 |
| Popis: | We have developed a model of oral ethanol ingestion in the ferret by providing a complete liquid diet in which 21% of the total caloric intake is given as ethanol. After 3 weeks of ethanol treatment, migration inhibitory factor activity from ferret lymphocytes was significantly decreased when compared to animals fed a dextrose-substituted, identical liquid diet. Lymphocyte blastogenesis in response to the mitogen, phytohemagglutinin, was also decreased after 6 weeks of ethanol ingestion. Histopathologically, hepatic cell degeneration, fat deposition, and "Mallory body-like" material were present after 11 weeks of therapy. Bone marrow aspirate cells from the iliac crest of ethanol-fed animals showed vacuolization in both erythroid and myeloid elements after 6 weeks of ethanol ingestion. Mean ferret weights were not significantly different between the ethanol and dextrose control groups; serum ethanol and acetaldehyde concentrations were somewhat higher over the study period compared to those seen in human alcoholics. Therefore, we have designed a small animal model of the toxic effects of alcohol in which multiple organ systems exhibited evidence of ethanol-related disease. |
| Databáze: | OpenAIRE |
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