ORF18 Is a Transfactor That Is Essential for Late Gene Transcription of a Gammaherpesvirus
| Autor: | Ren Sun, Hongyu Deng, Ting-Ting Wu, Vaithilingaraja Arumugaswami, Nichole Reyes, Qingmei Jia, DeeAnn Martinez-Guzman |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Gene Expression Regulation
Viral Time Factors Transcription Genetic viruses Immunology Molecular Sequence Data Genome Viral Biology Virus Replication Microbiology Virus Cell Line Mice Open Reading Frames Viral Proteins Plasmid Gammaherpesvirinae Virology Cricetinae Gene expression Animals Humans Amino Acid Sequence Antigens Promoter Regions Genetic Gene Conserved Sequence Regulation of gene expression Recombination Genetic Gene Expression Profiling virus diseases Genome Replication and Regulation of Viral Gene Expression Gene expression profiling Kinetics Viral replication Lytic cycle Insect Science Sequence Alignment Plasmids |
| Popis: | Lytic replication of the tumor-associated human gammaherpesviruses Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus has important implications in pathogenesis and tumorigenesis. Herpesvirus lytic genes have been temporally classified as exhibiting immediate-early (IE), early, and late expression kinetics. Though the regulation of IE and early gene expression has been studied extensively, very little is known regarding the regulation of late gene expression. Late genes, which primarily encode virion structural proteins, require viral DNA replication for their expression. We have identified a murine gammaherpesvirus 68 (MHV-68) early lytic gene, ORF18 , essential for viral replication. ORF18 is conserved in both beta- and gammaherpesviruses. By generating an MHV-68 ORF18-null virus, we characterized the stage of the virus lytic cascade that requires the function of ORF18. Gene expression profiling and quantitation of viral DNA synthesis of the ORF18-null virus revealed that the expression of early genes and viral DNA replication were not affected; however, the transcription of late genes was abolished. Hence, we have identified a gammaherpesvirus-encoded factor essential for the expression of late genes independently of viral DNA synthesis. |
| Databáze: | OpenAIRE |
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