Defining microbial biomarkers for risk of preterm labor

Autor: Rita de Cássia dos Santos Silveira, Andréa Lúcia Corso, Anderson Santos de Freitas, Priscila Caroline Thiago Dobbler, Renato S. Procianoy, Luiz Fernando Wurdig Roesch, Volker Mai
Rok vydání: 2019
Předmět:
Clinical Microbiology - Research Paper
Microbial DNA
Prevotella
Physiology
Cohort Studies
Medical microbiology
Pregnancy
Risk Factors
RNA
Ribosomal
16S
Lactobacillus
0303 health sciences
education.field_of_study
biology
Microbiota
Análise de sequência
Sequenciamento de nucleotídeos em larga escala
High-Throughput Nucleotide Sequencing
Vagina
Cohort
Nascimento prematuro
Female
Brazil
Adult
medicine.medical_specialty
Population
Vaginal microbiome
Microbiology
Young Adult
03 medical and health sciences
Obstetric Labor
Premature
Microbial ecology
Media Technology
medicine
Humans
Risk factor
education
030304 developmental biology
Fatores de risco
Bacteria
030306 microbiology
business.industry
biology.organism_classification
Biomarcadores
Brazilian microbiome
Next-generation sequencing
business
Biomarkers
Zdroj: Brazilian Journal of Microbiology
Repositório Institucional da UFRGS
Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
ISSN: 1678-4405
1517-8382
DOI: 10.1007/s42770-019-00118-x
Popis: Preterm birth remains the main contributor to early childhood mortality. The vaginal environment, including microbiota composition, might contribute to the risk of preterm delivery. Alterations in the vaginal microbial community structure might represent a risk factor for preterm birth. Here, we aimed to (a) investigate the association between preterm birth and the vaginal microbial community and (b) identify microbial biomarkers for risk of preterm birth. Microbial DNA was isolated from vaginal swabs in a cohort of 69 women enrolled at hospital admission for their delivery. Microbiota was analyzed by high-throughput 16S rRNA sequencing. While no differences in microbial diversity measures appeared associated with the spontaneous preterm and full-term outcomes, the microbial composition was distinct for these groups. Differential abundance analysis showed Lactobacillus species to be associated with full-term birth whereas an unknown Prevotella species was more abundant in the spontaneous preterm group. Although we studied a very miscegenated population from Brazil, our findings were similar to evidence pointed by other studies in different countries. The role of Lactobacillus species as a protector in the vaginal microbiome is demonstrated to be also a protector of spontaneous preterm outcome whereas the presence of pathogenic species, such as Prevotella spp., is endorsed as a factor of risk for spontaneous preterm delivery.
Databáze: OpenAIRE
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