Color-related chlorophyll and carotenoid concentrations of Chinese kale can be altered through CRISPR/Cas9 targeted editing of the carotenoid isomerase gene BoaCRTISO
| Autor: | Yan Wang, Fen Zhang, Hao Zheng, Bo Sun, Yue Jian, Yunting Zhang, Wenli Huang, Zheng Aihong, Qing Chen, Qiao Yuan, Haoru Tang, Yuanxiu Lin, Min Jiang, Qiaomei Wang, Xiaorong Wang |
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| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Genetics Transgenic plants Mutant Isomerase Gene food and beverages Molecular engineering in plants Plant Science Genetically modified crops Horticulture Biology Biochemistry Article chemistry.chemical_compound chemistry Chlorophyll Gene family CRISPR Secondary metabolism Carotenoid Gene Biotechnology |
| Zdroj: | Horticulture Research |
| ISSN: | 2052-7276 2662-6810 |
| DOI: | 10.1038/s41438-020-00379-w |
| Popis: | The carotenoid isomerase gene (BoaCRTISO) of Chinese kale was targeted and edited using the CRISPR/Cas9 system in the present study. The results showed a high mutation rate (81.25%), and 13 crtiso mutants were obtained. Only two types of mutations, insertions and replacements, were found. Both the total and individual carotenoid and chlorophyll concentrations of the biallelic and homozygous mutants were reduced, and the total levels declined by 11.89–36.33%. The color of the biallelic and homozygous mutants changed from green to yellow, likely reflecting a reduction in the color-masking effect of chlorophyll on carotenoids. The expression levels of most carotenoid and chlorophyll biosynthesis-related genes, including CRTISO, were notably lower in the mutants than in the WT plants. In addition, the functional differences between members of this gene family were discussed. In summary, these findings indicate that CRISPR/Cas9 is a promising technique for the quality improvement of Chinese kale and other Brassica vegetables. |
| Databáze: | OpenAIRE |
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