G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus
| Autor: | Caroline Mullier, Jean-Jacques Letesson, Michaël Deghelt, Géraldine Laloux, Nayla Francis, Xavier De Bolle, Christine Jacobs-Wagner, Delphine Dotreppe, Jean-François Sternon, Charles Van der Henst |
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| Přispěvatelé: | Department of Bio-engineering Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
DNA Replication
DNA Bacterial intracellular trafficking Applied Microbiology Population General Physics and Astronomy Brucella abortus Vacuole Biology Microbiology Article General Biochemistry Genetics and Molecular Biology Bacterial cell structure Brucellosis Bacterial genetics Caulobacter Genetics Humans education Molecular Biology Cells Cultured education.field_of_study Multidisciplinary Intracellular parasite Circular bacterial chromosome DNA replication cellular infection General Chemistry Cell cycle Chromosomes Bacterial bacterial cell cycle Virology Brucella G1 Phase Cell Cycle Checkpoints 3. Good health Vacuoles HeLa Cells |
| Zdroj: | Microbial Cell Deghelt, M, Mullier, C, Sternon, J-F, Francis, N, Laloux, G, Dotreppe, D, Van der Henst, C, Jacobs-Wagner, C, Letesson, J-J & De Bolle, X 2014, ' G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus ', Nature Communications, vol. 5, pp. 4366 . https://doi.org/10.1038/ncomms5366 Nature Communications |
| ISSN: | 2311-2638 |
| Popis: | Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localization of segregation and replication loci of the two bacterial chromosomes indicates that, immediately after being engulfed by host-cell endocytic vacuoles, most bacterial cells are newborn. These bacterial cells do not initiate DNA replication for the next 4 to 6 h, indicating a G1 arrest. Moreover, growth is completely stopped during that time, reflecting a global cell cycle block. Growth and DNA replication resume later, although bacteria still reside within endosomal-like compartments. We hypothesize that the predominance of G1-arrested bacteria in the infectious population, and the bacterial cell cycle arrest following internalization, may constitute a widespread strategy among intracellular pathogens to colonize new proliferation niches. Certain pathogenic bacteria such as Brucella abortus undergo a temporary non-proliferative stage after infecting host cells. Here, the authors study chromosomal replication and segregation in B. abortus during infection, showing that the non-proliferative stage consists of G1-arrested newborn cells. |
| Databáze: | OpenAIRE |
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