Phylogeny, Prevalence, and Shiga Toxin (Stx) Production of Clinical Escherichia coli O157 Clade 2 Strains Isolated in Shimane Prefecture, Japan
| Autor: | Eiji Yokoyama, Mayuko Kotani, Fumi Hayashi, Tomotake Sakai, Hiroshi Asakura, Jun Kawase, Yuta Kawakami, Aiko Fukuma, Morito Kurosaki, Shinichiro Hirai |
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| Rok vydání: | 2020 |
| Předmět: |
Linkage disequilibrium
Population Biology medicine.disease_cause Escherichia coli O157 Shiga Toxins Applied Microbiology and Biotechnology Microbiology Genetic analysis 03 medical and health sciences Japan Phylogenetics medicine Prevalence Humans Clade education Escherichia coli Escherichia coli Infections Phylogeny 030304 developmental biology Genetics 0303 health sciences education.field_of_study Phylogenetic tree 030306 microbiology Shiga toxin General Medicine biology.protein |
| Zdroj: | Current microbiology. 78(1) |
| ISSN: | 1432-0991 |
| Popis: | This study investigated the genetic and pathogenic variation of the subgroups of clade 2 strains of Shiga toxin (Stx)-producing Escherichia coli (STEC) O157. A total of 111 strains of STEC O157 isolated in Shimane prefecture, Japan, were classified in clade 2 (n = 39), clade 3 (n = 16), clade 4/5 (n = 3), clade 7 (n = 14), clade 8 (n = 17), and clade 12 (n = 22) by single-nucleotide polymorphism analysis and lineage-specific polymorphism assay-6. These results showed a distinct difference from our previous study in which clade 3 strains were the most prevalent strains in three other prefectures in Japan, indicating that the clade distribution of O157 strains was different in different geographic areas in Japan. Phylogenetic analysis using insertion sequence (IS) 629 distribution data showed that clade 2 strains formed two clusters, designated 2a and 2b. Stx2 production by cluster 2b strains was significantly higher than by cluster 2a strains (P |
| Databáze: | OpenAIRE |
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