Cardiovascular Risk Stratification Based on Oxidative Stress for Early Detection of Pathology
| Autor: | Gloria Alvarez-Llamas, Tamara Sastre-Oliva, Nerea Corbacho-Alonso, Paula J. Martinez, Luis M. Ruilope, Fernando Vivanco, E. Calvo, Luis Rodríguez Padial, Jesús Vázquez, Maria G. Barderas, Gema Ruiz-Hurtado, Elena Rodríguez-Sánchez, Juan Antonio López, M. Cabrera, Laura Mourino-Alvarez, Montserrat Baldan-Martin |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Proteome Physiology Enfermedad cardiovascular Clinical Biochemistry Glutathione reductase Disease Oxidative phosphorylation medicine.disease_cause Biochemistry 03 medical and health sciences Risk Factors Glutaredoxin Medicine Humans Cysteine Young adult Molecular Biology General Environmental Science 030102 biochemistry & molecular biology Adulto business.industry Estrés oxidativo Cell Biology Oxidative Stress Proteómica 030104 developmental biology Cardiovascular Diseases General Earth and Planetary Sciences Biomarker (medicine) business Oxidation-Reduction Oxidative stress |
| Zdroj: | Antioxidantsredox signaling. 35(8) |
| ISSN: | 1557-7716 |
| Popis: | Aims: Current cardiovascular (CV) risk prediction algorithms are able to quantify the individual risk of CV disease. However, CV risk in young adults is underestimated due to the high dependency of age in biomarker-based algorithms. Because oxidative stress is associated with CV disease, we sought to examine CV risk stratification in young adults based on oxidative stress to approach the discovery of new markers for early detection of pathology. Results: Young adults were stratified into (i) healthy controls, (ii) subjects with CV risk factors, and (iii) patients with a reported CV event. Plasma samples were analyzed using FASILOX, a novel approach to interrogate the dynamic thiol redox proteome. We also analyzed irreversible oxidation by targeted searches using the Uniprot database. Irreversible oxidation of cysteine (Cys) residues was greater in patients with reported CV events than in healthy subjects. These results also indicate that oxidation is progressive. Moreover, we found that glutathione reductase and glutaredoxin 1 proteins are differentially expressed between groups and are proteins involved in antioxidant response, which is in line with the impaired redox homeostasis in CV disease. Innovation: This study, for the first time, describes the oxidative stress (reversible and irreversible Cys oxidation) implication in human plasma according to CV risk stratification. Conclusion: The identification of redox targets and the quantification of protein and oxidative changes might help to better understand the role of oxidative stress in CV disease, and aid stratification for CV events beyond traditional prognostic and diagnostic markers. Instituto de Salud Carlos III PT13 / 0001/0013, PI14 / 01917, CP15 / 00129, PI17 / 01093, PI17 / 01193, FI18 / 00261, PI18 / 00995, y FI19 / 00157. Sociedad Española de Cardiología para la Investigación Básica, Beca PRB3 (IPT17 / 0019 - ISCIII-SGEFI / FEDER) 7.468 JCR (2021) Q1, 53/297 Biochemistry & Molecular Biology 1.832 SJR (2021) Q1, 42/439 Biochemistry No data IDR 2021 UEM |
| Databáze: | OpenAIRE |
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