Transcriptional foliar profile of the C3-CAM bromeliad Guzmania monostachia
| Autor: | Alejandra Matiz, Maria Aurineide Rodrigues, Luiz Lehmann Coutinho, Sónia C. S. Andrade, Bruno Nobuya Katayama Gobara, Paulo Tamaso Mioto, Ana Zangirolame Gonçalves, Helenice Mercier |
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| Rok vydání: | 2019 |
| Předmět: |
Chlorophyll
Pigments Bromeliaceae 0106 biological sciences 0301 basic medicine Leaves Chloroplasts De novo transcriptome assembly Gene Expression Plant Science Guzmania 01 natural sciences chemistry.chemical_compound Gene Expression Regulation Plant Photosynthesis BIOLOGIA MOLECULAR VEGETAL Materials Cellular Stress Responses Multidisciplinary Dehydration Gene Ontologies Plant Anatomy Trichome branching food and beverages Genomics Trichomes Droughts Cell Processes Physical Sciences Medicine Cellular Structures and Organelles Cellular Types Transcriptome Analysis Research Article Science Plant Cell Biology Materials Science Bromelia Biology Potassium ion import 03 medical and health sciences Plant Cells Botany Genetics Sequence Assembly Tools Organic Pigments Gene Expression Profiling fungi Biology and Life Sciences Computational Biology Water Cell Biology Genome Analysis biology.organism_classification Trichome Apex (geometry) Plant Leaves 030104 developmental biology chemistry Crassulacean acid metabolism Transcriptome 010606 plant biology & botany |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP PLoS ONE PLoS ONE, Vol 14, Iss 10, p e0224429 (2019) |
| Popis: | Guzmania monostachia is an epiphytic tank bromeliad that displays the inducible CAM photosynthesis under stressful conditions and had the highest stomata density in the leaf apex, while the base portion has the highest density of trichomes, which are specialized structures used to acquire water and nutrients from the tank solution. In order to correlate the genetic factors behind these morpho-physiological characteristics along the leaf blade of G. monostachia, a comparative transcriptome analysis was performed to identify the functional enriched pathways and unigenes that could play a role in the apical, middle and basal leaf portions. A total of 653 million reads were used for de novo transcriptome assembly, resulting in 48,051 annotated unigenes. Analysis of differentially expressed genes (DEGs) among distinct leaf regions revealed that 806 DEGs were upregulated in the apex compared to the middle portion, while 9685 DEGs were upregulated in the apex and 9784 DEGs were upregulated in the middle portions compared to the base. Our outcomes correlated some DEGs and identified unigenes with their physiological functions, mainly suggesting that the leaf apex was related to the regulation of stomatal movement, production of chlorophyll, cellular response to stress, and H2O2 catabolic process. In contrast, the middle portion showed DEGs associated with the transport of amino acids. Furthermore, DEGs from the leaf base were mainly correlated with responses to nutrients and nitrogen compounds, regulation of potassium ion import, response to water deprivation, and trichome branching, indicating that, at least in part, this leaf portion can replace some of the root functions of terrestrial plants. Therefore, possibly candidate unigenes and enriched pathways presented here could be prospected in future experimental work, opening new possibilities to bioengineer non-inducible CAM plants and/or improve the fertilization use efficiency by increasing leaf nutrient acquisition of crop plants. |
| Databáze: | OpenAIRE |
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