Phenotypic Characterization and RT-qPCR Analysis of Flower Development in F1 Transgenics of Chrysanthemum × grandiflorum
| Autor: | Muhammad Rashid Shaheen, Muhammad Ajmal Bashir, Saba Haider, Umer Habib, Fan Min, Yike Gao, Rashid Hussain |
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| Rok vydání: | 2021 |
| Předmět: |
hybrid identification
Genetics Regulation of gene expression Ecology Transgene fungi Botany food and beverages quantitative real time PCR Plant Science Genetically modified crops Biology Article RNA silencing RNA interference QK1-989 Gene expression cross breeding Gene silencing Gene Ecology Evolution Behavior and Systematics |
| Zdroj: | Plants Volume 10 Issue 8 Plants, Vol 10, Iss 1681, p 1681 (2021) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants10081681 |
| Popis: | Gene silencing is the epigenetic regulation of any gene in order to prevent gene expression at the transcription or translation levels. Among various gene silencing techniques, RNA silencing (RNAi) is notable gene regulation technique that involves sequence-specific targeting and RNA degradation. However, the effectiveness of transgene-induced RNAi in F1 generation of chrysanthemum has not been studied yet. In the current study, we used RNAi-constructed CmTFL1 (white-flowered) and CmSVP overexpressed (yellow flowered) transgenic plants of previously conducted two studies for our experiment. Cross hybridization was performed between these intergeneric transgenic and non-transgenic plants of the winter-growing chrysanthemum selection “37” (light pink flowered). The transgene CmSVP was confirmed in F1 hybrids by RT-PCR analysis, whereas hybrids of CmTFL1 parental plants were non-transgenic. Besides this, quantitative real-time PCR (qPCR) was used to explain the molecular mechanism of flower development using reference genes. Intergeneric and interspecific hybrids produced different colored flowers unlike their respective parents. These results suggest that generic traits of CmSVP overexpressed plants can be transferred into F1 generations when crossed with mutant plants. This study will aid in understanding the breeding phenomenon among intergeneric hybrids of chrysanthemum plants at an in vivo level, and such transgenics will also be more suitable for sustainable flower yield under a low-light production system. |
| Databáze: | OpenAIRE |
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