Chloromethane Metabolism by Methylobacterium sp. Strain CM4

Autor:	Thomas Leisinger, Alex Studer, Michael A. Kertesz, Todd Vannelli
Rok vydání:	1998
Předmět:	Ecology biology Methylamine Chloromethane Dehydrogenase Metabolism Physiology and Biotechnology biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Metabolic pathway chemistry Biochemistry Methylobacterium Formate Methanol Food Science Biotechnology
Zdroj:	Applied and Environmental Microbiology. 64:1933-1936
ISSN:	1098-5336 0099-2240
Popis:	Methylobacterium sp. strain CM4 metabolized chloromethane quantitatively with a molar yield of 2.8 g of whole-cell protein/mol of C. This value was similar to that observed after growth with methanol (2.9 g of protein/mol of C) and about three times larger than the yield with formate (0.94 g of protein/mol of C). Chloromethane dehalogenation activity was inducible. MiniTn 5 transposon insertion mutants with altered growth characteristics with chloromethane and other C 1 compounds were isolated and characterized. Nine of these were unable to grow with chloromethane but were able to grow with methanol, methylamine, or formate. Seventy-three transposon mutants that were defective in the utilization of either methanol, methylamine, methanol plus methylamine, or formate could still grow with chloromethane. Based on the protein yield data and the properties of the transposon mutants, we propose a pathway for chloromethane metabolism that depends on methyltransferase and dehydrogenase activities.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec9b0b6864b51357d79ccd7cc299cee5 https://doi.org/10.1128/aem.64.5.1933-1936.1998 Zobrazit plný text záznamu