Long-term lipid-based total parenteral nutrition activates mononuclear cells and modulates membrane lipid composition in pigs
| Autor: | Fredrik Müller, Tor-Arne Hagve, Peter Kierulf, Halvor Rollag, J Aksnes, K. Børsum |
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| Rok vydání: | 1995 |
| Předmět: |
Parenteral Nutrition
medicine.medical_specialty Necrosis Swine Linoleic acid Lymphocyte Acid Phosphatase Clinical Biochemistry Biology Lymphocyte Activation Neopterin Peripheral blood mononuclear cell Monocytes Membrane Lipids chemistry.chemical_compound Internal medicine Macrophages Alveolar medicine Animals Macrophage Lung Phospholipids Granuloma Nitroblue Tetrazolium Monocyte Fatty Acids General Medicine Macrophage Activation Biopterin Blood Coagulation Factors Endotoxins medicine.anatomical_structure Endocrinology chemistry Immunology Cytokines Muramidase Arachidonic acid Soybeans medicine.symptom Cell activation |
| Zdroj: | Scandinavian Journal of Clinical and Laboratory Investigation. 55:201-210 |
| ISSN: | 1502-7686 0036-5513 |
| DOI: | 10.3109/00365519509089614 |
| Popis: | In previous studies we have found lung granulomas in pigs on long-term soybean-based total parenteral nutrition (TPN). In the present study we have investigated activities and membrane lipids of cells known to participate in a granulomatous process. Pigs were given TPN with soybean emulsion for 7 weeks, controls were given saline intravenously and consumed a similar caloric load in a standard oral diet. Spontaneous nitroblue tetrazolium (NBT) reduction increased 204% in peripheral blood mononuclear cells (MNC) from TPN animals compared to controls (p < 0.05), and 161% (p = 0.05) in alveolar macrophages. The spontaneous lymphocyte mitogen response (LMR) rate in MNC increased 299% (p < 0.05). Endotoxin-stimulated procoagulant activity in MNC tended to increase in TPN animals. Acid phosphatase and lysozyme production in alveolar macrophages were not significantly changed. The serum neopterin level at the end of the observation was 7.0 nmol l-1 in TPN animals compared to 3.9 nmol l-1 in controls (p < 0.01), while interleukin-1 and tumour necrosis factor were not detectable. These findings indicated in vivo activation of peripheral blood monocytes, lymphocytes and alveolar macrophages in pigs on TPN. The membrane lipid contents of linoleic acid (18:2, n-6) increased, while arachidonic acid (20:4, n-6) tended to decrease both in MNC and lung tissue. Thus, linoleic acid conversion did not lead to accumulation of arachidonate in the cell membranes. Direct effects on monocytes, lymphocytes and alveolar macrophages of TPN particles, and altered cell membrane function, due to linoleic acid enrichment, are tentative explanations for the raised cell responses. Such cell activation may have contributed to the formation of granulomas. |
| Databáze: | OpenAIRE |
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