Correction to: Cytochrome P450 expression, induction and activity in human induced pluripotent stem cell-derived intestinal organoids and comparison with primary human intestinal epithelial cells and Caco-2 cells
| Autor: | Aafke W. F. Janssen, Loes P. M. Duivenvoorde, Deborah Rijkers, Rosalie Nijssen, Ad A. C. M. Peijnenburg, Meike van der Zande, Jochem Louisse |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Novel Foods & Agrochains BU Toxicologie Health Toxicology and Mutagenesis BU Contaminanten & Toxines Induced Pluripotent Stem Cells Team Toxicology Toxicology Novel Foods & Agroketens Gene Expression Regulation Enzymologic Cell Line BU Contaminants & Toxins Cytochrome P-450 Enzyme System Life Science Humans BU Toxicology Novel Foods & Agrochains Intestinal Mucosa Cells Cultured Cytochrome P-450 Enzyme Inducers BU Toxicology Correction Epithelial Cells General Medicine Team Pesticides 2 Organoids BU Toxicologie Novel Foods & Agroketens Female Caco-2 Cells |
| Zdroj: | Arch Toxicol Archives of Toxicology, 95(3), 923-923 Archives of Toxicology 95 (2021) 3 |
| ISSN: | 0340-5761 |
| Popis: | Human intestinal organoids (HIOs) are a promising in vitro model consisting of different intestinal cell types with a 3D microarchitecture resembling native tissue. In the current study, we aimed to assess the expression of the most common intestinal CYP enzymes in a human induced pluripotent stem cell (hiPSC)-derived HIO model, and the suitability of that model to study chemical-induced changes in CYP expression and activity. We compared this model with the commonly used human colonic adenocarcinoma cell line Caco-2 and with a human primary intestinal epithelial cell (IEC)-based model, closely resembling in vivo tissue. We optimized an existing protocol to differentiate hiPSCs into HIOs and demonstrated that obtained HIOs contain a polarized epithelium with tight junctions consisting of enterocytes, goblet cells, enteroendocrine cells and Paneth cells. We extensively characterized the gene expression of CYPs and activity of CYP3A4/5, indicating relatively high gene expression levels of the most important intestinal CYP enzymes in HIOs compared to the other models. Furthermore, we showed that CYP1A1 and CYP1B1 were induced by β-naphtoflavone in all three models, whereas CYP3A4 was induced by phenobarbital and rifampicin in HIOs, in the IEC-based model (although not statistically significant), but not in Caco-2 cells. Interestingly, CYP2B6 expression was not induced in any of the models by the well-known liver CYP2B6 inducer phenobarbital. In conclusion, our study indicates that hiPSC-based HIOs are a useful in vitro intestinal model to study biotransformation of chemicals in the intestine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink