Homogeneous mono-(125)i-insulins. Preparation and characterization of mono-(125)i-(tyr a14)-and mono-(125)i-(tyr a19)-insulin
| Autor: | K. H. Jørgensen, U. D. Larsen |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Macromolecular Substances
Swine Endocrinology Diabetes and Metabolism medicine.medical_treatment Insulin Antibodies Radioimmunoassay Antigen-Antibody Complex Iodine Radioisotopes chemistry.chemical_compound Drug Stability Oxidizing agent Internal Medicine medicine Animals Insulin Iodate Diiodotyrosine Chromatography Lactoperoxidase Monoiodotyrosine Biochemistry chemistry Homogeneous Intramolecular force Isotope Labeling |
| Zdroj: | Diabetologia. 19(6) |
| ISSN: | 0012-186X |
| Popis: | Mono-(125)I-(Tyr A19)-insulin (Mono*A19) was prepared by iodinating MC porcine insulin with (125)I in acid medium using iodate (as oxidizing agent), followed by anion-exchange chromatography. Mono-(125)I-(Tyr A14)-insulin (Mono*A14) was prepared by iodinating MC porcine insulin with (125)I, using H(2)O(2)/lactoperoxidase at neutral pH, followed by anion-exchange chromatography. The specific radioactivities were in the ranges of 120-200 and 220-300 mCi/mg for Mono*A19 and Mono*A14, respectively. Analyses of the intramolecular distributions of (125)I demonstrated that the preparations were 97-98% radiochemically pure. In both preparations, 98-99% of the radioactivity was capable of binding to insulin antibodies for up to 6 months of storage of the tracers. The IRI concentration decreased with the duration of storage. The greatest observed fall in IRI concentration was 70%. The ime course could be explained by the assumption that the disintegration of a (125)I-nucleus destroys the immunoreactivity of the insulin molecule in which the decay occurs. |
| Databáze: | OpenAIRE |
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