Effects of angiogenin on granulosa and theca cell function in cattle
| Autor: | J. L. Dentis, A. M. Burress, Nicole B. Schreiber, Leon J. Spicer |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Angiogenin Angiogenesis Granulosa cell angiogenin (ANG) SF1-1100 Article Andrology 03 medical and health sciences Follicle Ovarian Follicle Internal medicine medicine Animals Humans Granulosa cell proliferation Dose-Response Relationship Drug 030102 biochemistry & molecular biology urogenital system Chemistry IGF1 Theca Cell Ribonuclease Pancreatic theca cells Animal culture 030104 developmental biology Endocrinology granulosa cells Theca tumor necrosis factor α (TNFα) Cattle Female Animal Science and Zoology Folliculogenesis |
| Zdroj: | Animal, Vol 11, Iss 5, Pp 811-819 (2017) |
| ISSN: | 1751-7311 |
| DOI: | 10.1017/s1751731116002044 |
| Popis: | Angiogenin is a member of the ribonuclease A superfamily of proteins that has been implicated in stimulating angiogenesis but whether angiogenin can directly affect ovarian granulosa or theca cell function is unknown. Therefore, the objective of these studies was to determine the effect of angiogenin on proliferation and steroidogenesis of bovine granulosa and theca cells. In experiments 1 and 2, granulosa cells from small (1 to 5 mm diameter) follicles and theca cells from large (8 to 22 mm diameter) follicles were cultured to evaluate the dose-response effect of recombinant human angiogenin on steroidogenesis. At 30 and 100 ng/ml, angiogenin inhibited (P < 0.05) granulosa cell progesterone production and theca cell androstenedione production but did not affect (P > 0.10) granulosa cell estradiol production or theca cell progesterone production, and did not affect numbers of granulosa or theca cells. In experiments 3 and 4, granulosa and theca cells from both small and large follicles were cultured with 300 ng/ml of angiogenin to determine if size of follicle influenced responses to angiogenin. At 300 ng/ml, angiogenin increased large follicle granulosa cell proliferation but decreased small follicle granulosa cell progesterone and estradiol production and large follicle theca cell progesterone production. In experiments 5 and 6, angiogenin stimulated (P < 0.05) proliferation and DNA synthesis in large follicle granulosa cells. In experiment 7, 300 ng/ml of angiogenin increased (P < 0.05) CYP19A1 messenger RNA (mRNA) abundance in granulosa cells but did not affect CYP11A1 mRNA abundance in granulosa or theca cells and did not affect CYP17A1 mRNA abundance in theca cells. We conclude that angiogenin appears to target both granulosa and theca cells in cattle, but additional research is needed to further understand the mechanism of action of angiogenin in granulosa and theca cells, as well as its precise role in folliculogenesis. |
| Databáze: | OpenAIRE |
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