Environmental factors involved in axonal regeneration following spinal cord transection in rats
| Autor: | Tsukasa Kanchiku, Yoshihiko Kato, Toshihiko Taguchi, Hideo Kataoka, Satoru Yoshii, Hidenori Suzuki, Toshikazu Gondo, Takahiro Yara |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Pathology and Forensic Medicine Implants Experimental Neurotrophic factors Internal medicine medicine Glial cell line-derived neurotrophic factor Animals Nerve Growth Factors Axon Molecular Biology Spinal cord injury Spinal Cord Injuries biology Guided Tissue Regeneration Regeneration (biology) General Medicine Anatomy Spinal cord medicine.disease Rats Inbred F344 Nerve Regeneration Rats medicine.anatomical_structure Nerve growth factor Endocrinology nervous system Astrocytes biology.protein Female Collagen Immunostaining |
| Zdroj: | Medical molecular morphology. 42(3) |
| ISSN: | 1860-1499 |
| Popis: | A recent study of a rat model treated with grafted collagen filament (CF) after spinal cord transection showed dramatic recovery of motor function but did not report on the acute-stage phenomenon. In the present study, we describe molecular and histological aspects of the axonal regeneration process during the acute stage following spinal cord transection. The spinal cord of 8-week-old rats was completely transected, and a scaffold of almost the same size as the resected portion was implanted in the gap. Changes in the mRNA expression of four neurotrophic factors [nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), NT-3, and glial cell-derived neurotrophic factor (GDNF)] were analyzed after 72 h. The expression of BDNF and NT-3 mRNA increased significantly in the CF-grafted group compared to the nongrafted group. Immunostaining for BDNF and NT-3 revealed that cells positive for these neurotrophic factors extended along the collagen filaments in the CF-grafted group. Similarly, astrocytes extended into the collagen filament scaffold together with the neurotrophic factors and partly across a border line. These findings indicate that collagen filament helps to reduce scar tissue, supports the expression of neurotrophic factors, and serves as a scaffold for the outgrowth of regenerating axons. |
| Databáze: | OpenAIRE |
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