Localized in Vivo Model Drug Delivery with Intravascular Ultrasound and Microbubbles

Autor: Douglas K. Bowles, Brian R. Wamhoff, Alexander L. Klibanov, John A. Hossack, Joseph P. Kilroy
Rok vydání: 2014
Předmět:
Zdroj: Ultrasound in Medicine & Biology. 40:2458-2467
ISSN: 0301-5629
DOI: 10.1016/j.ultrasmedbio.2014.04.007
Popis: An intravascular ultrasound (IVUS) and microbubble drug delivery system was evaluated in both ex vivo and in vivo swine vessel models. Microbubbles with the fluorophore DiI embedded in the shell as a model drug were infused into ex vivo swine arteries at a physiological flow rate (105 mL/min) while a 5 MHz IVUS transducer applied ultrasound. Ultrasound pulse sequences consisted of acoustic radiation force pulses to displace DiI-loaded microbubbles from the vessel lumen to the wall followed by higher intensity delivery pulses to release DiI into the vessel wall. Insonation with both the acoustic radiation force pulse and the delivery pulse increased DiI deposition 10-fold compared to deposition with the delivery pulse alone. Localized delivery of DiI was then demonstrated in an in vivo swine model. The theoretical transducer beam width predicted the measured angular extent of delivery to within 11%. These results demonstrate that low frequency IVUS catheters are a viable method for achieving localized drug delivery with microbubbles.
Databáze: OpenAIRE