Emergence of Enterohemorrhagic Escherichia coli Serovar O157 Strains in Clade 8 with Highly Similar Pulsed-Field Gel Electrophoresis Patterns
| Autor: | Eiji Yokoyama, Yoshiki Etoh, Yuko Matsumoto, Kazumi Horikawa, Kiyoe Yoda, Noriko Konishi, Sachiko Ichihara, Hitomi Kasahara, Morito Kurosaki, Takayuki Kurazono, Akemi Kai |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Serotype clone (Java method) Lineage (genetic) Biology Escherichia coli O157 medicine.disease_cause Polymorphism Single Nucleotide Microbiology Linkage Disequilibrium Japan Pulsed-field gel electrophoresis medicine Humans Clade Escherichia coli Gene Escherichia coli Infections Gel electrophoresis Genetics Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Genes Bacterial Food Microbiology Food Science |
| Zdroj: | Journal of Food Protection. 74:1324-1327 |
| ISSN: | 1944-9097 0362-028X |
| Popis: | Enterohemorrhagic Escherichia coli serovar O157 (O157) strains with highly similar pulsed-field gel electrophoresis (PFGE) patterns were isolated in Japan during 2007 and 2008. Several genetic features related to O157 evolution were investigated to indicate whether homoplasy might have contributed to the highly similar PFGE patterns in these strains. The O157 strains were classified in lineage I/II, as defined by a lineage-specific polymorphism assay-6 with an atypical allele in Z5935 (code: 231111). Analysis of the insertion sites of stx2 phage in these strains showed that the sites were “occupied” in yehV and “intact” in wrbA, indicating that the strains were derived from “Cluster 1” of “Subgroup C.” When a specific single-nucleotide polymorphism in ECs2357 in clade 8 strains was investigated, all of the strains in the present study were confirmed to be clade 8 strains. These results indicated that the O157 strains in this study had common genetic features, suggesting that the highly similar PFGE patterns of these strains were not due to homoplasy. Because no common source of these strains could be identified in 2007 to 2008 in Japan, these strains may have emerged from a unique O157 clade 8 clone and then spread by dissemination in Japan. |
| Databáze: | OpenAIRE |
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