Cryopreservation of sievers wormwood (Artemisia sieversiana Ehrh. Ex Willd.) seeds by vitrification and encapsulation
| Autor: | Dong-Jin Park, Mi Jin Jeong, Seong Hyeon Yong, Cheul Ho Lee, Myung Suk Choi, Eunji Choi, Woo Hyeong Yang, Gang Uk Suh, Yuwon Seol |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Artemisia sieversiana
ttc staining 0211 other engineering and technologies food and beverages 021107 urban & regional planning Forestry 02 engineering and technology 010501 environmental sciences Management Monitoring Policy and Law Biology cryopreservation 01 natural sciences Cryopreservation vitrification Staining Area at risk Horticulture germination Germination encapsulation lcsh:SD1-669.5 Vitrification lcsh:Forestry 0105 earth and related environmental sciences |
| Zdroj: | Forest Science and Technology, Vol 15, Iss 4, Pp 180-186 (2019) |
| ISSN: | 2158-0715 2158-0103 |
| Popis: | Artemisia sieversiana is being used for the medicinal purpose, there are not many conservation objects, and the species is registered as a rare plant in Korea. There is also no research on the preservation of A. sieversiana seeds. In this paper, we investigated the preservation of seeds using cryopreservation. The vitality and germination rate of seeds were investigated with pretreatment, vitrification, and encapsulation. The initial germination rate of the seeds was 95%. PVS3 solution treated for 60 min showed the highest vitality, and germination rate. When encapsulation, both vitality and germination rate decreased. Changes in vitality rate during storage period were measured. As a result, the seed-maintained vitality when the vitrification solution used. Encapsulation is unavailable because of too much change. Therefore, seeds can be safely preserved without deterioration of vitality at cryopreservation by using PVS 3 solution and it will be helpful in future studies. |
| Databáze: | OpenAIRE |
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