Lack of RUNX3 regulation in human gastric cancer
| Autor: | Mathias J Friedrich, Roland M. Schmid, Rupert Langer, Christian Prinz, Petra Voland, H Hoefler, Markus Gerhard, Roland Rad |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Tumor suppressor gene Biopsy Down-Regulation Adenocarcinoma Helicobacter Infections Pathology and Forensic Medicine Stroma Stomach Neoplasms Leukocytes medicine Humans Neoplastic transformation RNA Messenger RNA Neoplasm Stomach cancer Microdissection Aged Neoplasm Staging Laser capture microdissection Aged 80 and over Helicobacter pylori biology Reverse Transcriptase Polymerase Chain Reaction digestive oral and skin physiology Cancer Middle Aged biology.organism_classification medicine.disease digestive system diseases Neoplasm Proteins Gene Expression Regulation Neoplastic Cell Transformation Neoplastic Core Binding Factor Alpha 3 Subunit Gastric Mucosa Female |
| Zdroj: | The Journal of Pathology. 210:141-146 |
| ISSN: | 1096-9896 0022-3417 |
| DOI: | 10.1002/path.2042 |
| Popis: | It has been proposed that the transcription factor RUNX3 is the product of a gastric tumour suppressor gene. We examined RUNX3 expression in gastric biopsies from 105 patients with different histological presentations. Surprisingly, immunohistochemical staining detected RUNX3 protein expression only in infiltrating leukocytes but not in the gastric epithelium. Using laser capture microdissection and quantitative reverse transcription-polymerase chain reaction, we confirmed that the level of RUNX3 mRNA expression in the gastric epithelium was very low and was influenced neither by H. pylori infection nor by neoplastic transformation. Instead, RUNX3 was highly expressed in the gastric stroma and the level of expression correlated with the magnitude of H. pylori-induced gastric inflammation. The low level of RUNX3 expression in gastric epithelium and the absence of downregulation in gastric cancer do not support the hypothesis that RUNX3 functions as a gastric tumour suppressor gene. |
| Databáze: | OpenAIRE |
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