Biofabrication of functional protein nanoparticles through simple His-tag engineering
| Autor: | Neus Ferrer-Miralles, Eric Voltà-Durán, Verónica Nolan, Nerea Roher, Laura Sánchez-García, Mauricio Rojas-Peña, Ugutz Unzueta, Julieta M. Sánchez, Jose Vicente Carratalá, Alejandro Sánchez-Chardi, Naroa Serna, Ario de Marco, Antonio Villaverde, Hèctor López-Laguna, Esther Vázquez, Sandra S. Flores, Eloi Parladé, Olivia Cano-Garrido |
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| Rok vydání: | 2021 |
| Předmět: |
Divalent cations
Materials science Renewable Energy Sustainability and the Environment Functional protein General Chemical Engineering Protein species Nanoparticle Nanotechnology General Chemistry Environmentally friendly Protein materials Simple (abstract algebra) Nanoparticles Environmental Chemistry Protein engineering Biomaterials design Microscale chemistry Research Article Biofabrication |
| Zdroj: | ACS Sustainable Chemistry & Engineering r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| ISSN: | 2168-0485 |
| DOI: | 10.1021/acssuschemeng.1c04256 |
| Popis: | We have developed a simple, robust, and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in a spontaneous and reproducible protein assembly as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembly of these materials is not linked to particular polypeptide features, and it is based on an environmentally friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, are architecturally stable, are fully functional, and serve as intermediates in a more complex assembly process, resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforwardly applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats. A simple, cost-effective, and green method is proposed to generate functional, protein-only nanoparticles with clinical applicability out of any desired polypeptide. |
| Databáze: | OpenAIRE |
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