Changes in ceramide levels upon catechins-induced apoptosis in LoVo cells
| Autor: | Bo Jiang, Yali Zhang, Xiaohua Tan, Dianyuan Zhou |
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| Rok vydání: | 2002 |
| Předmět: |
Base line
Diacylglycerol Kinase Ceramide Chemistry food and beverages Apoptosis General Medicine Ceramides Apoptotic death complex mixtures Catechin General Biochemistry Genetics and Molecular Biology Cell biology Kinetics chemistry.chemical_compound Colonic Neoplasms Tumor Cells Cultured Humans heterocyclic compounds sense organs General Pharmacology Toxicology and Pharmaceutics Intracellular Lovo cell |
| Zdroj: | Life Sciences. 70:2023-2029 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(01)01543-0 |
| Popis: | It has been demonstrated that there is difference in the induction of apoptosis in LoVo cells by (−)-epigallocatechin-3-gallate (EGCG), (−)-epigallocatechin (EGC), and (−)-epicatechin (EC). In this study, we explored changes in ceramide levels upon the three catechins-induced apoptosis in LoVo cells. Addition of C 2 - and C 6 -ceramide to LoVo cells mimicked EGCG or EGC in leading to apoptotic death. Further measurement of intracellular ceramide content showed that the treatment of LoVo cells with EGCG or EGC resulted in a rapidly transient increase in ceramide content, and then back gradually to base line level, whereas the action of EC was just opposite to that of EGCG or EGC. These results suggest there is difference in the generation of intracellular ceramide by the three catechins and ceramide may take part in the regulation of EGCG- or EGC-induced apoptosis in LoVo cells. |
| Databáze: | OpenAIRE |
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