Periodate degradation of labeled ascorbic acid

Autor: Bert M. Tolbert, Larry M. Plunkett, Ronald J. Harkrader
Rok vydání: 1976
Předmět:
Zdroj: Analytical Biochemistry. 72:310-314
ISSN: 0003-2697
Popis: The degradation of ascorbic acid by periodate has been studied using [1-14C] or [6-14C]ascorbic acid, as well as quantitative yield of the various products. Ascorbic acid, oxidized at pH 7.5 is cleaved to give 2 mol formate, 1 mol CO2, 1 mol of oxalate, and 1 mol formaldehyde. The oxalate and formaldehyde are derived from the 1- and 6-carbons, respectively. About 17% of C1 is oxidized to CO2 by a side reaction. Dehydroascorbic acid gives the same degradation products as ascorbic acid. Detailed procedures for the degradation are presented.
Databáze: OpenAIRE