Periodate degradation of labeled ascorbic acid
| Autor: | Bert M. Tolbert, Larry M. Plunkett, Ronald J. Harkrader |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Oxalates
Formates Periodic Acid Biophysics Side reaction Formaldehyde Periodate Ascorbic Acid Cell Biology Ascorbic acid Dehydroascorbic Acid Biochemistry Oxalate chemistry.chemical_compound chemistry Yield (chemistry) Methods Organic chemistry Formate Dehydroascorbic acid Carbon Radioisotopes Oxidation-Reduction Molecular Biology Nuclear chemistry |
| Zdroj: | Analytical Biochemistry. 72:310-314 |
| ISSN: | 0003-2697 |
| Popis: | The degradation of ascorbic acid by periodate has been studied using [1-14C] or [6-14C]ascorbic acid, as well as quantitative yield of the various products. Ascorbic acid, oxidized at pH 7.5 is cleaved to give 2 mol formate, 1 mol CO2, 1 mol of oxalate, and 1 mol formaldehyde. The oxalate and formaldehyde are derived from the 1- and 6-carbons, respectively. About 17% of C1 is oxidized to CO2 by a side reaction. Dehydroascorbic acid gives the same degradation products as ascorbic acid. Detailed procedures for the degradation are presented. |
| Databáze: | OpenAIRE |
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