Relationship of human herpesvirus 8 peripheral blood virus load and Kaposi's sarcoma clinical stage
| Autor: | Thomas B. Campbell, Lisa Fitzpatrick, Ivy Gudza, Robert T. Schooley, Lovemore Gwanzura, Samantha MaWhinney, Irene E. White, Buxton Ndemera, Margaret Borok |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Zimbabwe viruses Immunology HIV Infections Biology Virus Replication Peripheral blood mononuclear cell law.invention Pathogenesis law Immunopathology medicine Humans Immunology and Allergy Sarcoma Kaposi Kaposi's sarcoma Polymerase chain reaction virus diseases Middle Aged Viral Load medicine.disease Virology Cross-Sectional Studies Infectious Diseases DNA Viral Herpesvirus 8 Human HIV-1 Leukocytes Mononuclear biology.protein Female Viral disease Antibody Viral load |
| Zdroj: | AIDS. 14:2109-2116 |
| ISSN: | 0269-9370 |
| DOI: | 10.1097/00002030-200009290-00006 |
| Popis: | Objective: To determine the relationship between human herpesvirus 8 (HHV-8 or Kaposi's sarcoma-associated herpesvirus) peripheral blood virus load and Kaposi's sarcoma (KS) clinical stage. Design: Blinded, cross-sectional analysis of peripheral blood HHV-8 DNA levels in persons with AIDS-related KS in Harare, Zimbabwe. Methods: Subjects were stratified by KS clinical stage. The amount of HHV-8 DNA in plasma and peripheral blood mononuclear cells (PBMC) was determined by quantitative real-time PCR amplification of the HHV-8 open reading frame 26. Results: Thirty-one HIV-1/HHV-8-coinfected persons were studied: 26 subjects had histologically confirmed KS (one stage II, 11 stage III and 14 stage IV) and five subjects had antibodies to HHV-8 but did not have KS. The age, CD4 lymphocyte count and plasma HIV-1 RNA levels were similar in all groups. HHV-8 DNA was detected in the plasma of all HHV-8-infected subjects (range < 2.4 to 5.2 log 10 copies/ml), but plasma HHV-8 DNA levels were not associated with KS disease stage. In contrast, the amount of HHV-8 DNA in PBMC (range < 0.7 to 4.5 log 10 copies/μg) was strongly associated with KS clinical stage (P= 0.005). Among stage IV KS cases, there was a linear relationship between plasma and PBMC HHV-8 DNA levels (r 2 = 0.42; P= 0.01). Conclusion: The strong association observed between the extent of KS disease and the levels of HHV-8 DNA in PBMC provides further evidence for a relationship between HHV-8 virus load and KS pathogenesis. |
| Databáze: | OpenAIRE |
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