Hydrolytic stability and biocompatibility on smooth muscle cells of polyethylene glycol-polycaprolactone-based polyurethanes

Autor: Said Arévalo-Alquichire, Maria Morales-Gonzalez, Manuel F. Valero, Juan Angel Sans, José A. Gómez-Tejedor, Luis E. Díaz, Guillermo Vilariño-Feltrer
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Polyethylene glycol-polycaprolactone
Materials science
Biocompatibility
09.- Desarrollar infraestructuras resilientes
promover la industrialización inclusiva y sostenible
y fomentar la innovación
02 engineering and technology
Polyethylene glycol
010402 general chemistry
01 natural sciences
Pentaerythritol
chemistry.chemical_compound
Hydrolysis
Smooth muscle
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
General Materials Science
Hydrolytic stability
Mechanical Engineering
Biomaterial
Adhesion
musculoskeletal system
021001 nanoscience & nanotechnology
Condensed Matter Physics
Polyurethan
0104 chemical sciences
12.- Garantizar las pautas de consumo y de producción sostenibles
chemistry
Mechanics of Materials
FISICA APLICADA
Polycaprolactone
MAQUINAS Y MOTORES TERMICOS
cardiovascular system
Biophysics
0210 nano-technology
Zdroj: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
Popis: [EN] Interactions between smooth muscle cells (SMCs) and biomaterials must not result in phenotype changes as this may generate uncontrolled multiplication processes and occlusions in vascular grafts. The aim of this study was to relate the hydrolytic stability and biocompatibility of polyurethanes (PUs) on SMCs. A higher polycaprolactone (PCL) concentration was found to improve the hydrolytic stability of the material and the adhesion of SMCs. A material with 5% polyethylene glycol, 90% PCL, and 5% pentaerythritol presented high cell viability and adhesion, suggesting a contractile phenotype in SMCs depending on the morphology. Nevertheless, all PUs retained their elastic modulus over 120 days, similar to the collagen of native arteries (similar to 10 MPa). Furthermore, aortic SMCs did not present toxicity (viability over 80%) and demonstrated adherence without any abnormal cell multiplication processes, which is ideal for the function to be fulfiled in situ in the vascular grafts.
The research and publication were supported by the Universidad de La Sabana (ING-205-2018) and the Minister of Science, Technology, and Innovation of the Republic of Colombia, MINCIENCAS (Contract number 80740-186-2019). M. M-G. would like to thank the Universidad de La Sabana for the scholarship for her master's studies. S. A-A. would like to thank MINCIENCIAS for the doctoral training scholarship (Grant 727-2015). The authors are thankful to Professor Ericsson Coy Barrera and his staff at Nueva Granada Military University for the access to the VarioskanT LUX multimode microplate reader. J. A. S. acknowledges the financial support by MINECO through FIS2017-83295-P, MAT2015-71070-REDC, MAT2016-75586-C4-1/2/3-P and the Ramon y Cajal Fellowship (RYC-201517482). CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.
Databáze: OpenAIRE
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