Infection, replication, and cytopathology of human papillomavirus type 31 in trophoblasts
| Autor: | Yong Liu, Nalini Agrawal, Paul L. Hermonat, C. Krishna Prasad, Hong You, Maurizio Chiriva-Internati, Helen H. Kay, Curtis L. Lowery |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA Replication
Human papillomavirus L1 Cell Count Biology Transfection Virus Replication Virus Cell Line chemistry.chemical_compound Virology medicine Cell Adhesion Humans Receptor Cell adhesion Papillomaviridae reproductive and urinary physiology Reverse Transcriptase Polymerase Chain Reaction Trophoblast virus diseases Oncogene Proteins Viral Molecular biology female genital diseases and pregnancy complications Trophoblasts medicine.anatomical_structure chemistry embryonic structures biology.protein Receptors Virus Antibody DNA |
| Zdroj: | Virology. 316(2):281-289 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2003.08.020 |
| Popis: | Human papillomavirus (HPV) DNA is preferentially found in spontaneous abortions, specifically residing in trophoblasts, and transfected HPV-16 DNA replicates and produces progeny in 3A trophoblasts in culture. In this study 3A trophoblasts were shown to display both HPV receptors and infection by HPV-31b and HPV-6 virus resulted in de novo (increasing) HPV DNA replication in these cells (inhibited by neutralizing anti-HPV31b antibodies). Reverse transcription-polymerase chain reaction analysis revealed that E1^E4, E6, and L1 were significantly expressed at days 5 (early) and 10 (late), respectively, and in situ immunocytochemistry verified L1 protein expression. Perhaps most important, HPV 31b virus infection caused both a decrease in 3A trophoblast cell numbers in a dose-dependent manner and a low trophoblast-endometrial cell adhesion (both inhibited by neutralizing anti-HPV-31 antibodies). These data further support the hypothesis that HPVs are fully active in trophoblasts and may cause some spontaneous abortions. |
| Databáze: | OpenAIRE |
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