Agreement between ELISA and plaque reduction neutralisation assay in Detection of respiratory syncytial virus specific antibodies in a birth Cohort from Kilifi, coastal Kenya
| Autor: | J. Anthony G. Scott, D. James Nokes, Joyce U. Nyiro, Patrick K. Munywoki, Patience K. Kiyuka, Charles J. Sande, Martin Mutunga |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
viruses Medicine (miscellaneous) Respiratory syncytial virus General Biochemistry Genetics and Molecular Biology Neutralization Virus Immunoglobulin G Serology 03 medical and health sciences 0302 clinical medicine Antigen Medicine specific antibody serological assays 030212 general & internal medicine Respiratory system Neutralizing antibody biology business.industry virus diseases Articles biochemical phenomena metabolism and nutrition Virology digestive system diseases 3. Good health 030104 developmental biology biology.protein birth cohort Antibody business agreement Research Article RC |
| Zdroj: | Wellcome Open Research |
| ISSN: | 2398-502X |
| DOI: | 10.12688/wellcomeopenres.15108.1 |
| Popis: | Background: Severe disease associated with respiratory syncytial virus (RSV) infection occurs predominantly among infants under 6 months of age. Vaccines for prevention are in clinical development. Assessment of the vaccine effectiveness in large epidemiological studies requires serological assays which are rapid, economical and standardised between laboratories. The objective of this study was to assess the agreement between two enzyme linked immunosorbent assays (ELISA) and the plaque reduction neutralisation test (PRNT) in quantifying RSV specific antibodies. Methods: Archived sera from 99 participants of the Kilifi Birth Cohort (KBC) study (conducted 2002-2007) were screened for RSV antibodies using 3 methods: ELISA using crude RSV lysate as antigen, a commercial RSV immunoglobulin G (IgG) ELISA kit from IBL International GmbH, and PRNT. Pearson correlation, Bland-Altman plots and regression methods were used in analysis. Results: There was high positive correlation between the IBL RSV IgG ELISA and PRNT antibodies (Pearson r=0.75), and moderate positive correlation between the crude RSV lysate IgG ELISA and PRNT antibodies (r= 0.61). Crude RSV lysate IgG ELISA showed a wider 95% limit of agreement (-1.866, 6.157) with PRNT compared to the IBL RSV IgG ELISA (1.392, 7.595). Mean PRNT titres were estimated within a width of 4.8 log2PRNT and 5.6 log2PRNT at 95% prediction interval by IBL RSV IgG and crude RSV lysate IgG ELISA, respectively. Conclusion: Although, the IBL RSV IgG ELISA is observed to provide a reasonable correlate for PRNT assay in detecting RSV specific antibodies, it does not provide an accurate prediction for neutralizing antibody levels. An RSV neutralising antibody level is likely to fall within 2.4 fold higher and 2.4 fold lower than the true value if IBL RSV IgG ELISA is used to replace PRNT assay. The utility of an ELISA assay in vaccine studies should be assessed independent of the PRNT method. |
| Databáze: | OpenAIRE |
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