Sepsis and Hemocyte Loss in Honey Bees (Apis mellifera) Infected with Serratia marcescens Strain Sicaria
| Autor: | Eric Neeno-Eckwall, James O. Church, David M. Warshauer, Nancy L. Burritt, Nicole T. Perna, Anna M. Hilger, Nicole J. Foss, Jacob A. Hildebrand, James B. Burritt |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serratia Hemocytes lcsh:Medicine Insect Pathology and Laboratory Medicine Winter cluster White Blood Cells Animal Cells Hemolymph RNA Ribosomal 16S Invertebrate Genomics Medicine and Health Sciences lcsh:Science Phylogeny Serratia marcescens media_common Mites Multidisciplinary Serratia infection biology Genomics Bees Bacterial Pathogens Insects Phenotype Medical Microbiology behavior and behavior mechanisms Pathogens Cellular Types Honey Bees Research Article animal structures Arthropoda media_common.quotation_subject Immune Cells 030106 microbiology Immunology Microbiology Serratia Infections 03 medical and health sciences Enterobacteriaceae Sepsis medicine Genetics Animals Microbial Pathogens Blood Cells Bacteria lcsh:R fungi Organisms Biology and Life Sciences Computational Biology Honey bee Sequence Analysis DNA Cell Biology Comparative Genomics biology.organism_classification medicine.disease Invertebrates Hymenoptera 030104 developmental biology Animal Genomics Varroa destructor lcsh:Q Genome Bacterial |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 12, p e0167752 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Global loss of honey bee colonies is threatening the human food supply. Diverse pathogens reduce honey bee hardiness needed to sustain colonies, especially in winter. We isolated a free-living Gram negative bacillus from hemolymph of worker honey bees (Apis mellifera) found separated from winter clusters. In some hives, greater than 90% of the dying bees detached from the winter cluster were found to contain this bacterium in their hemolymph. Throughout the year, the same organism was rarely found in bees engaged in normal hive activities, but was detected in about half of Varroa destructor mites obtained from colonies that housed the septic bees. Flow cytometry of hemolymph from septic bees showed a significant reduction of plasmatocytes and other types of hemocytes. Interpretation of the16S rRNA sequence of the bacterium indicated that it belongs to the Serratia genus of Gram-negative Gammaproteobacteria, which has not previously been implicated as a pathogen of adult honey bees. Complete genome sequence analysis of the bacterium supported its classification as a novel strain of Serratia marcescens, which was designated as S. marcescens strain sicaria (Ss1). When compared with other strains of S. marcescens, Ss1 demonstrated several phenotypic and genetic differences, including 65 genes not previously found in other Serratia genomes. Some of the unique genes we identified in Ss1 were related to those from bacterial insect pathogens and commensals. Recovery of this organism extends a complex pathosphere of agents which may contribute to failure of honey bee colonies. |
| Databáze: | OpenAIRE |
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