A Net Mold-Based Method of Biomaterial-Free Three-Dimensional Cardiac Tissue Creation
| Autor: | Chin Siang Ong, Bai Yang, Anjana Jeyaram, Takahiro Inoue, Isaree Pitaktong, Gordon F. Tomaselli, Steven M. Jay, Cecillia Lui, Zayneb Mohamed, Leslie Tung, Deborah DiSilvestre, Hiroshi Matsushita, Chunye Ma, Narutoshi Hibino, Enoch Yeung |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Cell Survival Biomedical Engineering Medicine (miscellaneous) Biocompatible Materials Bioengineering Exosomes Cell Line Electrocardiography Rats Nude Spheroids Cellular Internal medicine medicine Animals Humans Ligation Cell Size Ischemic cardiomyopathy Tissue Engineering business.industry Biomaterial Heart Arteries medicine.disease Alternative treatment Rats Rats Inbred Lew Irreversible loss Heart failure Cardiology Female Collagen business |
| Zdroj: | Tissue Engineering Part C: Methods. 25:243-252 |
| ISSN: | 1937-3392 1937-3384 |
| Popis: | Ischemic cardiomyopathy poses a significant public health burden due to the irreversible loss of functional cardiac tissue. Alternative treatment strategies include creation of three-dimensional (3D) cardiac tissues to both replace and augment injured native tissue. In this study, we utilize a net mold-based method to create a biomaterial-free 3D cardiac tissue and compare it to current methods using biomaterials. Cardiomyocytes, fibroblasts, and endothelial cells were combined using a hanging drop method to create spheroids. For the net mold patch method, spheroids were seeded into a net mold-based system to create biomaterial-free 3D cardiac patches. For the gel patch, spheroids were embedded in a collagen gel. Immunohistochemistry revealed increased alignment, vascularization, collagen I expression, cell viability, and higher density of cells in the net mold patch compared with the gel patch. Furthermore |
| Databáze: | OpenAIRE |
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